Involvement of tumor-associated macrophage activation in vitro during development of a novel mantle cell lymphoma cell line, PF-1, derived from a typical patient with relapsed disease

Abstract Human mantle cell lymphoma (MCL) cell lines are scarce and have been only sporadically described and validated, and only a few have been thoroughly molecularly or genetically characterized. We describe here the successful establishment of a new MCL line, PF-1, with typical MCL characteristi...

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Bibliographic Details
Published in:Leukemia & lymphoma 2015-01, Vol.56 (1), p.186-193
Main Authors: Pham, Lan V., Vang, Muychi T., Tamayo, Archito T., Lu, Gary, Challagundla, Pramoda, Jorgensen, Jeffrey L., Rollo, Alex A., Ou, Zhishuo, Zhang, Liang, Wang, Michael, Ford, Richard J.
Format: Article
Language:English
Subjects:
Antigens, Surface - metabolism
Cell Line, Tumor
Chromosome Aberrations
Humans
Immunophenotyping
In Situ Hybridization, Fluorescence
in vitro microenvironment
Lymphoma, Mantle-Cell - genetics
Lymphoma, Mantle-Cell - immunology
Lymphoma, Mantle-Cell - pathology
Macrophage Activation - immunology
macrophages
Macrophages - immunology
Macrophages - metabolism
Macrophages - pathology
Mantle cell lymphoma cell line
Neoplasm Recurrence, Local
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Summary:Abstract Human mantle cell lymphoma (MCL) cell lines are scarce and have been only sporadically described and validated, and only a few have been thoroughly molecularly or genetically characterized. We describe here the successful establishment of a new MCL line, PF-1, with typical MCL characteristics. Culturing primary MCL cells in vitro initially gave rise to an essential generative microenvironment "niche" involving macrophages required for MCL growth, and eventually produced the PF-1 MCL cell line. Our analysis revealed that PF-1 is morphologically and genotypically nearly identical to the original tumor cells. The PF-1 MCL cell line that we have developed will be useful for in vitro and in vivo studies of MCL pathogenesis and therapeutics.
ISSN:1042-8194
1029-2403
DOI:10.3109/10428194.2014.901511