Optimal dosing regimen of nitric oxide donor compounds for the reduction of Pseudomonas aeruginosa biofilm and isolates from wastewater membranes

Membrane fouling by bacterial biofilms remains a key challenge for membrane-based water purification systems. Here, the optimal biofilm dispersal potential of three nitric oxide (NO) donor compounds, viz. sodium nitroprusside, 6-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-hexanamine (MAHMA NO...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2013-01, Vol.29 (2), p.203-212
Main Authors: Barnes, Robert J., Bandi, Ratnaharika R., Wong, Wee Seng, Barraud, Nicolas, McDougald, Diane, Fane, Anthony, Kjelleberg, Staffan, Rice, Scott A.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Bacteria
Bacterial Load
biofilm prevention
Biofilms
Biofilms - drug effects
Biofouling
Biofouling - prevention & control
Bioreactors
Cells
Confocal microscopy
Data processing
Deoxyribonucleic acid
Dispersal
Dispersion
DNA
EPS
Extracellular
Hydrazines - pharmacology
L-Proline
Membrane processes
Membranes
Membranes, Artificial
Microbial Sensitivity Tests - methods
Microbial Viability
Microscopy
Microscopy, Confocal
mixed species biofilms
Nitric oxide
Nitric Oxide Donors - pharmacology
Nitroprusside - pharmacology
Optimization
Polysaccharides
Polysaccharides, Bacterial - isolation & purification
Proline
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - isolation & purification
Pseudomonas aeruginosa - physiology
Purification
Reduction
Reverse osmosis
Saccharides
Sodium
Sodium nitroprusside
Time Factors
Waste Water - microbiology
Wastewater
Water purification
Water Purification - instrumentation
Water Purification - methods
Water treatment
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Summary:Membrane fouling by bacterial biofilms remains a key challenge for membrane-based water purification systems. Here, the optimal biofilm dispersal potential of three nitric oxide (NO) donor compounds, viz. sodium nitroprusside, 6-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-hexanamine (MAHMA NONOate) and 1-(hydroxy-NNO-azoxy)-L-proline, disodium salt, was investigated using Pseudomonas aeruginosa PAO1 as a model organism. Dispersal was quantitatively assessed by confocal microscopy [bacterial cells and the components of the extracellular polymeric substances (EPS) (polysaccharides and extracellular DNA)] and colony-forming unit counts. The three NO donor compounds had different optimal exposure times and concentrations, with MAHMA NONOate being the optimal NO donor compound. Biofilm dispersal correlated with a reduction in both bacterial cells and EPS. MAHMA NONOate also reduced single species biofilms formed by bacteria isolated from industrial membrane bioreactor and reverse osmosis membranes, as well as in isolates combined to generate mixed species biofilms. The data present strong evidence for the application of these NO donor compounds for prevention of biofouling in an industrial setting.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2012.760069