Enhanced MHC class I and costimulatory molecules on B16F10 cells by Ganoderma lucidum polysaccharides

Purpose: It is obvious that malignant cells evade from immune system in patients with manifest malignancy. Deficient major histocompatibility complex (MHC) class I and costimulatory molecules on malignant cells partially consist of evasion strategy since antigen bond MHC and costimulatory molecules...

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Bibliographic Details
Published in:Journal of drug targeting 2012-08, Vol.20 (7), p.582-592
Main Authors: Sun, Li-Xin, Lin, Zhi-Bin, Duan, Xin-Suo, Lu, Jie, Ge, Zhi-Hua, Li, Xue-Fei, Li, Xue-Jun, Li, Min, Xing, En-Hong, Song, You-Xin, Jia, Jing, Li, Wei-Dong
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents, Phytogenic - chemistry
Antineoplastic Agents, Phytogenic - therapeutic use
B7-1 Antigen - biosynthesis
B7-2 Antigen - biosynthesis
Biological and medical sciences
Cell Proliferation - drug effects
Cell Survival - drug effects
costimulatory molecule
Ganoderma lucidum polysaccharides
Gene Expression Regulation, Neoplastic - drug effects
Gene Expression Regulation, Neoplastic - genetics
General pharmacology
Genes, MHC Class I - drug effects
Genes, MHC Class I - genetics
H-2 Antigens - biosynthesis
Medical sciences
Melanoma, Experimental - drug therapy
MHC class I
Mice
Mice, Inbred C57BL
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Polysaccharides - chemistry
Polysaccharides - therapeutic use
Reishi - chemistry
tumor
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Summary:Purpose: It is obvious that malignant cells evade from immune system in patients with manifest malignancy. Deficient major histocompatibility complex (MHC) class I and costimulatory molecules on malignant cells partially consist of evasion strategy since antigen bond MHC and costimulatory molecules provide two signals necessary for T cell activation. Therefore, enhancement of MHC-I and costimulatory molecules may favor restraint of the evasion. For this purpose, Ganoderma lucidum Polysaccharides (Gl-PS) was used on B16F10 melanoma cells in this study. Methods: Immunocytochemistry and flowcytometry were used to determine the H-2Kb and H-2Db (two prominent MHC class I molecules in C57BL mouse) as well as B7-1 and B7-2 (two prominent costimulatory molecules) expression on B16F10 cells after incubation with Gl-PS, while messenger ribonucleic acid (mRNA) of these molecules was detected by reverse transcription polymerase chain reaction (RT-PCR). Results: The H-2Kb and H-2Db, and B7-1 and B7-2 on B16F10 cells and mRNAs of these molecules were enhanced by Gl-PS, and more efficient antitumor cytotoxicity was induced by the Gl-PS treated cells. Conclusions: The MHC class I molecules and costimulatory molecules may be enhanced by Gl-PS, and more efficient immune cell mediated cytotoxicity against these B16F10 cells may be induced, which may favor cancer therapy.
ISSN:1061-186X
1029-2330
DOI:10.3109/1061186X.2012.697167