In vivo measurement of the shape of the tissue-refractive-index correlation function and its application to detection of colorectal field carcinogenesis

Polarization-gated spectroscopy is an established method to depth-selectively interrogate the structural properties of biological tissue. We employ this method in vivo in the azoxymethane (AOM)-treated rat model to monitor the morphological changes that occur in the field of a tumor during early car...

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Bibliographic Details
Published in:Journal of biomedical optics 2012-04, Vol.17 (4), p.047005
Main Authors: Gomes, Andrew J, Ruderman, Sarah, DelaCruz, Mart, Wali, Ramesh K, Roy, Hemant K, Backman, Vadim
Format: Article
Language:English
Subjects:
Algorithms
Animals
Azoxymethane
Case-Control Studies
Colonic Neoplasms - chemically induced
Colonic Neoplasms - chemistry
Colonic Neoplasms - diagnosis
Colonoscopy
Male
Monte Carlo Method
Neoplasms, Experimental - chemically induced
Neoplasms, Experimental - chemistry
Neoplasms, Experimental - diagnosis
Rats
Rats, Inbred F344
Refractometry - instrumentation
Refractometry - methods
Spectrum Analysis - instrumentation
Spectrum Analysis - methods
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Summary:Polarization-gated spectroscopy is an established method to depth-selectively interrogate the structural properties of biological tissue. We employ this method in vivo in the azoxymethane (AOM)-treated rat model to monitor the morphological changes that occur in the field of a tumor during early carcinogenesis. The results demonstrate a statistically significant change in the shape of the refractive-index correlation function for AOM-treated rats versus saline-treated controls. Since refractive index is linearly proportional to mass density, these refractive-index changes can be directly linked to alterations in the spatial distribution patterns of macromolecular density. Furthermore, we found that alterations in the shape of the refractive-index correlation function shape were an indicator of both present and future risk of tumor development. These results suggest that noninvasive measurement of the shape of the refractive-index correlation function could be a promising marker of early cancer development.
ISSN:1560-2281
DOI:10.1117/1.JBO.17.4.047005