sRNA-Xcc1, an integron-encoded transposon- and plasmid-transferred trans-acting sRNA, is under the positive control of the key virulence regulators HrpG and HrpX of Xanthomonas campestris pathovar campestris

sRNA-Xcc1 is a trans-acting sRNA recently identified from the plant pathogenic bacterium Xanthomonas campestris pathovar campestris (Xcc). Here, the phylogenetic distribution, predicted secondary structure and regulation of expression of sRNA-Xcc1 were analyzed. The analysis showed (1) a total 81 sR...

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Bibliographic Details
Published in:RNA biology 2011-11, Vol.8 (6), p.947-953
Main Authors: Chen, Xiao-Lin, Tang, Dong-Jie, Jiang, Rui-Ping, He, Yong-Qiang, Jiang, Bo-Le, Lu, Guang-Tao, Tang, Ji-Liang
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Base Sequence
Binding
Biology
Bioscience
Blotting, Northern
Calcium
Cancer
Cell
Cycle
DNA Transposable Elements - genetics
Gene Expression Regulation, Bacterial
Integrons - genetics
Landes
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Organogenesis
Phylogeny
Plasmids - genetics
Promoter Regions, Genetic - genetics
Proteins
RNA Families
RNA, Bacterial - chemistry
RNA, Bacterial - classification
RNA, Bacterial - genetics
RNA, Small Untranslated - chemistry
RNA, Small Untranslated - classification
RNA, Small Untranslated - genetics
Sequence Homology, Nucleic Acid
Trans-Activators - genetics
Transcription Factors - genetics
Virulence - genetics
Xanthomonas campestris - genetics
Xanthomonas campestris - pathogenicity
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Summary:sRNA-Xcc1 is a trans-acting sRNA recently identified from the plant pathogenic bacterium Xanthomonas campestris pathovar campestris (Xcc). Here, the phylogenetic distribution, predicted secondary structure and regulation of expression of sRNA-Xcc1 were analyzed. The analysis showed (1) a total 81 sRNA-Xcc1 homologs that are found in some bacterial strains that are taxonomically unrelated, belonging to the a-, b-, g-, and d-proteobacteria (2) that some sRNA-Xcc1 homologs are located in a plasmid-borne transposon or near a transposase coding gene, (3) that sRNA-Xcc1 is encoded by a integron gene cassette in Xcc and sRNA-Xcc1 homologs occur in integron gene cassettes of some uncultured bacteria and (4) that sRNA-Xcc1 homologs have a highly conserved sequence motif and a stable consensus secondary structure. These findings strongly support the idea that sRNA-Xcc1 represents a novel family of sRNAs which may be originally captured by integrons from natural environments and then spread among different bacterial species via horizontal gene transfer, possibly by means of transposons and plasmids. The expression analysis results demonstrated that the transcription of sRNA-Xcc1 is under the positive control of the key virulence regulators HrpG and HrpX, indicating that sRNA-Xcc1 may be involved in the virulence regulation of Xcc.
ISSN:1547-6286
1555-8584
DOI:10.4161/rna.8.6.16690