Isolation and characterization of lipoteichoic acid, a cell envelope component involved in preventing phage adsorption, from Lactococcus lactis subsp. cremoris SK110

The cell envelope of the phage-resistant Lactococcus lactis subsp. cremoris SK110 differed from its phage-sensitive variant by the presence of a galactosyl-containing component. This component was present in material obtained from SK110 by a mild alkali treatment. In a similar fraction extracted fro...

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Bibliographic Details
Published in:Journal of Bacteriology 1990-12, Vol.172 (12), p.7126-7130
Main Authors: Sijtsma, L. (ATO Agrotechnological Research Institute, Wageningen, The Netherlands), Wouters, J.T.M, Hellingwerf, K.J
Format: Article
Language:English
Subjects:
ADSORCION
ADSORPTION
BACTERIOFAGOS
Bacteriology
BACTERIOPHAGE
Bacteriophages - growth & development
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Galactose - analysis
Hydrogen-Ion Concentration
Immunodiffusion
Lactococcus lactis - analysis
Lectins - metabolism
Lipopolysaccharides - chemistry
Lipopolysaccharides - isolation & purification
Microbiology
Morphology, structure, chemical composition
PARED CELULAR
PAROI CELLULAIRE
POLISACARIDOS
POLYHOLOSIDE
STREPTOCOCCUS LACTIS
Teichoic Acids - chemistry
Teichoic Acids - isolation & purification
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Summary:The cell envelope of the phage-resistant Lactococcus lactis subsp. cremoris SK110 differed from its phage-sensitive variant by the presence of a galactosyl-containing component. This component was present in material obtained from SK110 by a mild alkali treatment. In a similar fraction extracted from SK112, no galactosyl-containing components were detected. With respect to gel permeation chromatography and electrophoretic mobility, identical characteristics of the alkali-extracted material and purified lipoteichoic acid (LTA) were measured. Chemical analysis of the latter component showed the absence of galactose in LTA isolated from SK112, whereas it was present in LTA obtained from SK110. In this paper, we propose that galactosyl-containing LTA is involved in preventing phage adsorption to L. lactis subsp. cremoris SK110
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.172.12.7126-7130.1990