Transglutaminase: New insights into gelatin nanoparticle cross-linking

Gelatin nanoparticles (GNPs) have demonstrated to be beneficial as a biodegradable and biocompatible delivery system. So far, nanoparticles prepared by the two-step desolvation technique were subsequently cross-linked by glutaraldehyde to guarantee storage stability. Although in vivo and in vitro to...

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Bibliographic Details
Published in:Journal of microencapsulation 2010-12, Vol.27 (8), p.747-754
Main Authors: Fuchs, S., Kutscher, M., Hertel, T., Winter, G., Pietzsch, M., Coester, C.
Format: Article
Language:English
Subjects:
Acetone
Animals
Biological and medical sciences
Catalysis
cross-linking
Cross-Linking Reagents - chemistry
drug delivery
Drug Stability
Drug Storage
Escherichia coli - enzymology
Gelatin
Gelatin - chemistry
General pharmacology
Glutaral - chemistry
Medical sciences
nanoparticle
Nanoparticles - chemistry
Nephelometry and Turbidimetry
Particle Size
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Solvents
stability
Swine
Temperature
Transglutaminases - chemistry
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Summary:Gelatin nanoparticles (GNPs) have demonstrated to be beneficial as a biodegradable and biocompatible delivery system. So far, nanoparticles prepared by the two-step desolvation technique were subsequently cross-linked by glutaraldehyde to guarantee storage stability. Although in vivo and in vitro toxicological studies have not revealed any glutaraldehyde related undesired effects, an alternative to chemical cross-linking could ease future clinical use in humans. Therefore, the recombinant enzyme microbial transglutaminase was used to examine its cross-linking abilities in nanoparticle production. Various process parameters, such as incubation time, temperature, medium, pH and the particle purification were evaluated regarding their impact on particle size and its distribution. Cross-linking reactions were best at 25°C using an ion-free solvent at a neutral pH and have been terminated after 12 h. Preliminary storage stability testing indicated adequate consistency of particle size and particle distribution making transglutaminase a potential candidate for glutaraldehyde substitution in future GNP production.
ISSN:0265-2048
1464-5246
DOI:10.3109/02652048.2010.518773