Exploring the aneugenic and clastogenic potential in the nanosize range: A549 human lung carcinoma cells and amorphous monodisperse silica nanoparticles as models

Abstract We explored how to assess the genotoxic potential of nanosize particles with a well validated assay, the in vitro cytochalasin-B micronucleus assay, detecting both clastogens and aneugens. Monodisperse Stöber amorphous silica nanoparticles (SNPs) of three different sizes (16, 60 and 104 nm)...

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Published in:Nanotoxicology 2010-12, Vol.4 (4), p.382-395
Main Authors: Gonzalez, Laetitia, Thomassen, Leen C. J., Plas, Gina, Rabolli, Virginie, Napierska, Dorota, Decordier, Ilse, Roelants, Mathieu, Hoet, Peter H., Kirschhock, Christine E. A., Martens, Johan A., Lison, Dominique, Kirsch-Volders, Micheline
Format: Article
Language:English
Subjects:
Amorphous silica nanoparticles
Analysis of Variance
aneuploidy
Cell Line, Tumor
Chromosomes
Dose-Response Relationship, Drug
dosimetry
genotoxicity
Humans
Light
Lung Neoplasms - metabolism
Models, Chemical
Mutagenicity Tests - methods
Nanoparticles - chemistry
Nanoparticles - toxicity
Particle Size
Scattering, Radiation
Silicon Dioxide - chemistry
Silicon Dioxide - pharmacokinetics
Silicon Dioxide - toxicity
size
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Summary:Abstract We explored how to assess the genotoxic potential of nanosize particles with a well validated assay, the in vitro cytochalasin-B micronucleus assay, detecting both clastogens and aneugens. Monodisperse Stöber amorphous silica nanoparticles (SNPs) of three different sizes (16, 60 and 104 nm) and A549 lung carcinoma cells were selected as models. Cellular uptake of silica was monitored by ICP-MS. At non-cytotoxic doses the smallest particles showed a slightly higher fold induction of micronuclei (MNBN). When considering the three SNPs together, particle number and total surface area appeared to account for MNBN induction as they both correlated significantly with the amplitude of the effect. Using nominal or cellular dose did not show statistically significant differences. Likewise, alkaline comet assay and FISH-centromeric probing of MNBN indicated a weak and not statistically significant induction of oxidative DNA damage, chromosome breakage and chromosome loss. This line of investigation will contribute to adequately design and interpret nanogenotoxicity assays.
ISSN:1743-5390
1743-5404
DOI:10.3109/17435390.2010.501913