High-Cell-Density Cyclic Fed-Batch Fermentation of a Poly(3-Hydroxybutyrate)-Accumulating Thermophile, Chelatococcus sp. Strain MW10

Different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium, Chelatococcus sp. strain MW10, with the aim of achieving high-cell-density (HCD) growth and high poly(3-hydroxybutyrate) [poly(3HB)] productivity. Enhanced cultiv...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2010-12, Vol.76 (23), p.7890-7895
Main Authors: Ibrahim, Mohammad H.A, Steinbüchel, Alexander
Format: Article
Language:English
Subjects:
Bacteria
Batch culture
batch fermentation
Beijerinckiaceae - growth & development
Beijerinckiaceae - metabolism
Biological and medical sciences
Bioreactors - microbiology
Biotechnology
Cell cycle
Cell growth
Cells
Chelatococcus
Culture Media - chemistry
Fermentation
Fundamental and applied biological sciences. Psychology
Hydroxybutyrates - metabolism
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microbiology
Polyesters - metabolism
polyhydroxybutyrate
thermophilic bacteria
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Summary:Different fermentation strategies were employed for the cultivation of a new poly(3-hydroxybutyrate)-accumulating thermophilic bacterium, Chelatococcus sp. strain MW10, with the aim of achieving high-cell-density (HCD) growth and high poly(3-hydroxybutyrate) [poly(3HB)] productivity. Enhanced cultivation was achieved by a cyclic fed-batch fermentation (CFBF) technique (42-liter scale). Maximal poly(3HB) productivity was obtained during the second cycle [16.8 ± 4.2 g poly(3HB)/liter]. At the end of CFBF (265 h), an HCD of up to 115.0 ± 4.3 g cell dry weight/liter was achieved.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.01488-10