Pronounced Effect of the Nature of the Inoculum on Biofilm Development in Flow Systems

Biofilm formation renders sessile microbial populations growing in continuous-flow systems less susceptible to variation in dilution rate than planktonic cells, where dilution rates exceeding an organism's maximum growth rate (μmax) results in planktonic cell washout. In biofilm-dominated syste...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2010-09, Vol.76 (18), p.6025-6031
Main Authors: Kroukamp, Otini, Dumitrache, Romeo G, Wolfaardt, Gideon M
Format: Article
Language:English
Subjects:
Biofilms
Biofilms - growth & development
Biological and medical sciences
Carbon Dioxide - metabolism
Cells
Colony Count, Microbial
Fundamental and applied biological sciences. Psychology
Genotype & phenotype
Green Fluorescent Proteins - metabolism
Immunization
Methods
Microbiology
Microorganisms
Plankton
Pseudomonas - growth & development
Pseudomonas aeruginosa
Pseudomonas fluorescens
Species Specificity
Studies
Time Factors
Water Movements
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Summary:Biofilm formation renders sessile microbial populations growing in continuous-flow systems less susceptible to variation in dilution rate than planktonic cells, where dilution rates exceeding an organism's maximum growth rate (μmax) results in planktonic cell washout. In biofilm-dominated systems, the biofilm's overall μmax may therefore be more relevant than the organism's μmax, where the biofilm μmax is considered as a net process dependent on the adsorption rate, growth rate, and removal rate of cells within the biofilm. Together with lag (acclimation) time, the biofilm's overall μmax is important wherever biofilm growth is a dominant form, from clinical settings, where the aim is to prevent transition from lag to exponential growth, to industrial bioreactors, where the aim is to shorten the lag and rapidly reach maximum activity. The purpose of this study was to measure CO₂ production as an indicator of biofilm activity to determine the effect of nutrient type and concentration and of the origin of the inoculum on the length of the lag phase, biofilm μmax, and steady-state metabolic activity of Pseudomonas aeruginosa PA01 (containing gfp), Pseudomonas fluorescens CT07 (containing gfp), and a mixed community. As expected, for different microorganisms the lengths of the lag phase in biofilm development and the biofilm μmax values differ, whereas different nutrient concentrations result in differences in the lengths of lag phase and steady-state values but not in biofilm μmax rates. The data further showed that inocula from different phenotypic origins give rise to lag time of different lengths and that this influence persists for a number of generations after inoculation.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.00070-10