Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients

Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H 2 O 2 , which is continuously released by neuronal cells. In vivo, the production of not...

Full description

Saved in:
Bibliographic Details
Published in:Redox report : communications in free radical research 2010-04, Vol.15 (2), p.81-86
Main Authors: Spasojević, Ivan, Stević, Zorica, Nikolić-Kokić, Aleksandra, Jones, David R., Blagojević, Duško, Spasić, Mihajlo B.
Format: Article
Language:English
Subjects:
AMYOTROPHIC LATERAL SCLEROSIS
Amyotrophic Lateral Sclerosis - metabolism
ASCORBATE
Ascorbic Acid - metabolism
Cerebrospinal Fluid - metabolism
Electron Spin Resonance Spectroscopy
EPR
Free Radical Scavengers - metabolism
Humans
IRON
URATE
Uric Acid - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H 2 O 2 , which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H 2 O 2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.
ISSN:1351-0002
1743-2928
DOI:10.1179/174329210X12650506623320