Loading…
Determination of phytoestrogens in dietary supplements by LC-MS/MS
Labelling data quantifying the exact content of individual phytoestrogen analytes in dietary supplements are generally poor. As these products are commonly used in the management of menopause symptoms, any clinical benefits would be dependent on the exact dosage of isoflavones received. Well-establi...
Saved in:
| Published in: | Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2008-05, Vol.25 (5), p.534-547 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c562t-7018c4eb07761f38a0a2881c67bd57e638bad11710094a203f0b5e92f532cad63 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c562t-7018c4eb07761f38a0a2881c67bd57e638bad11710094a203f0b5e92f532cad63 |
| container_end_page | 547 |
| container_issue | 5 |
| container_start_page | 534 |
| container_title | Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment |
| container_volume | 25 |
| creator | Clarke, D. B. Bailey, V. Lloyd, A. S. |
| description | Labelling data quantifying the exact content of individual phytoestrogen analytes in dietary supplements are generally poor. As these products are commonly used in the management of menopause symptoms, any clinical benefits would be dependent on the exact dosage of isoflavones received. Well-established extraction procedures and updated isotope dilution mass spectrometry liquid chromatography coupled with tandem mass spectrometry detection (LC-MS/MS) have been used to accurately quantify the concentrations of ten common isoflavones in 35 dietary supplement samples on sale in the UK, Canada and Italy. Concentration-specific ionization suppression is described for biochanin A and formononetin. All supplements contained phytoestrogens. The soya isoflavones (genistein, daidzein, glycitein) were present in all products and the majority also contained the red clover isoflavones (biochanin A, formononetin) and some the Kudzu isoflavones (daidzein, puerarin). The content of total isoflavones per dose ranged from |
| doi_str_mv | 10.1080/02652030701658340 |
| format | article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmed_primary_18478479</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18478479</sourcerecordid><originalsourceid>FETCH-LOGICAL-c562t-7018c4eb07761f38a0a2881c67bd57e638bad11710094a203f0b5e92f532cad63</originalsourceid><addsrcrecordid>eNqFkM1KxDAUhYMojo4-gBvpC9S5SZqfghsdf2EGF6PrkraJVtqkJBm0b29lBl0MIly4d3G-w7kHoTMMFxgkzIBwRoCCAMyZpBnsoSOcZ1kKwMT-z53lE3QcwjsAJwLnh2iCZSbGyY_Q9Y2O2neNVbFxNnEm6d-G6HSI3r1qG5LGJnWjo_JDEtZ93-pO2xiSckgW83S5mi1XJ-jAqDbo0-2eope72-f5Q7p4un-cXy3SinES0zGkrDJdghAcGyoVKCIlrrgoayY0p7JUNcYCA-SZGt8yUDKdE8MoqVTN6RThjW_lXQhem6L3TTcGKzAU330UO32MzPmG6ddlp-tfYlvAKBAbQWON8536cL6ti6iG1nnjla2asGtbxM84kpf_kvTvZF9bV378</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Determination of phytoestrogens in dietary supplements by LC-MS/MS</title><source>Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Science and Technology Collection (Reading list)</source><creator>Clarke, D. B. ; Bailey, V. ; Lloyd, A. S.</creator><creatorcontrib>Clarke, D. B. ; Bailey, V. ; Lloyd, A. S.</creatorcontrib><description>Labelling data quantifying the exact content of individual phytoestrogen analytes in dietary supplements are generally poor. As these products are commonly used in the management of menopause symptoms, any clinical benefits would be dependent on the exact dosage of isoflavones received. Well-established extraction procedures and updated isotope dilution mass spectrometry liquid chromatography coupled with tandem mass spectrometry detection (LC-MS/MS) have been used to accurately quantify the concentrations of ten common isoflavones in 35 dietary supplement samples on sale in the UK, Canada and Italy. Concentration-specific ionization suppression is described for biochanin A and formononetin. All supplements contained phytoestrogens. The soya isoflavones (genistein, daidzein, glycitein) were present in all products and the majority also contained the red clover isoflavones (biochanin A, formononetin) and some the Kudzu isoflavones (daidzein, puerarin). The content of total isoflavones per dose ranged from <1 to 53 mg. Trace amounts of coumestrol were found in six products. Other less common analytes, the prenylnaringenins (6-prenylnaringenin, 8-prenylnaringenin, 6,8-diprenylnaringenin) were not found in any of the products. Only 14 of 35 supplements were found to deliver more than or equal to 40 mg day
−1
of aglycone isoflavones, a consensus dose value recognized as delivering therapeutic benefit. Eleven did not match label claims. Six delivered less than 10 mg day
−1
of isoflavones. There has been little improvement in the overall quality of industry labelling in the five years since this was last investigated. Consequently, the public, retailers and healthcare professionals should consider using standardized isoflavone supplements, which are supported by analytical measurements.</description><identifier>ISSN: 1944-0049</identifier><identifier>EISSN: 1944-0057</identifier><identifier>DOI: 10.1080/02652030701658340</identifier><identifier>PMID: 18478479</identifier><language>eng</language><publisher>England: Taylor & Francis Group</publisher><subject>Chromatography, Liquid - methods ; dietary supplements ; Dietary Supplements - analysis ; Female ; Glycine max - chemistry ; Humans ; isoflavone intake ; Isoflavones - administration & dosage ; Isoflavones - analysis ; Isoflavones - chemistry ; menopause ; Menopause - drug effects ; Phytoestrogens ; Phytoestrogens - administration & dosage ; Phytoestrogens - analysis ; Phytoestrogens - chemistry ; Reference Standards ; soya, red clover ; Tandem Mass Spectrometry - methods ; Trifolium - chemistry</subject><ispartof>Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 2008-05, Vol.25 (5), p.534-547</ispartof><rights>Copyright Taylor & Francis Group, LLC 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-7018c4eb07761f38a0a2881c67bd57e638bad11710094a203f0b5e92f532cad63</citedby><cites>FETCH-LOGICAL-c562t-7018c4eb07761f38a0a2881c67bd57e638bad11710094a203f0b5e92f532cad63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18478479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clarke, D. B.</creatorcontrib><creatorcontrib>Bailey, V.</creatorcontrib><creatorcontrib>Lloyd, A. S.</creatorcontrib><title>Determination of phytoestrogens in dietary supplements by LC-MS/MS</title><title>Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment</title><addtitle>Food Addit Contam Part A Chem Anal Control Expo Risk Assess</addtitle><description>Labelling data quantifying the exact content of individual phytoestrogen analytes in dietary supplements are generally poor. As these products are commonly used in the management of menopause symptoms, any clinical benefits would be dependent on the exact dosage of isoflavones received. Well-established extraction procedures and updated isotope dilution mass spectrometry liquid chromatography coupled with tandem mass spectrometry detection (LC-MS/MS) have been used to accurately quantify the concentrations of ten common isoflavones in 35 dietary supplement samples on sale in the UK, Canada and Italy. Concentration-specific ionization suppression is described for biochanin A and formononetin. All supplements contained phytoestrogens. The soya isoflavones (genistein, daidzein, glycitein) were present in all products and the majority also contained the red clover isoflavones (biochanin A, formononetin) and some the Kudzu isoflavones (daidzein, puerarin). The content of total isoflavones per dose ranged from <1 to 53 mg. Trace amounts of coumestrol were found in six products. Other less common analytes, the prenylnaringenins (6-prenylnaringenin, 8-prenylnaringenin, 6,8-diprenylnaringenin) were not found in any of the products. Only 14 of 35 supplements were found to deliver more than or equal to 40 mg day
−1
of aglycone isoflavones, a consensus dose value recognized as delivering therapeutic benefit. Eleven did not match label claims. Six delivered less than 10 mg day
−1
of isoflavones. There has been little improvement in the overall quality of industry labelling in the five years since this was last investigated. Consequently, the public, retailers and healthcare professionals should consider using standardized isoflavone supplements, which are supported by analytical measurements.</description><subject>Chromatography, Liquid - methods</subject><subject>dietary supplements</subject><subject>Dietary Supplements - analysis</subject><subject>Female</subject><subject>Glycine max - chemistry</subject><subject>Humans</subject><subject>isoflavone intake</subject><subject>Isoflavones - administration & dosage</subject><subject>Isoflavones - analysis</subject><subject>Isoflavones - chemistry</subject><subject>menopause</subject><subject>Menopause - drug effects</subject><subject>Phytoestrogens</subject><subject>Phytoestrogens - administration & dosage</subject><subject>Phytoestrogens - analysis</subject><subject>Phytoestrogens - chemistry</subject><subject>Reference Standards</subject><subject>soya, red clover</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Trifolium - chemistry</subject><issn>1944-0049</issn><issn>1944-0057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkM1KxDAUhYMojo4-gBvpC9S5SZqfghsdf2EGF6PrkraJVtqkJBm0b29lBl0MIly4d3G-w7kHoTMMFxgkzIBwRoCCAMyZpBnsoSOcZ1kKwMT-z53lE3QcwjsAJwLnh2iCZSbGyY_Q9Y2O2neNVbFxNnEm6d-G6HSI3r1qG5LGJnWjo_JDEtZ93-pO2xiSckgW83S5mi1XJ-jAqDbo0-2eope72-f5Q7p4un-cXy3SinES0zGkrDJdghAcGyoVKCIlrrgoayY0p7JUNcYCA-SZGt8yUDKdE8MoqVTN6RThjW_lXQhem6L3TTcGKzAU330UO32MzPmG6ddlp-tfYlvAKBAbQWON8536cL6ti6iG1nnjla2asGtbxM84kpf_kvTvZF9bV378</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Clarke, D. B.</creator><creator>Bailey, V.</creator><creator>Lloyd, A. S.</creator><general>Taylor & Francis Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20080501</creationdate><title>Determination of phytoestrogens in dietary supplements by LC-MS/MS</title><author>Clarke, D. B. ; Bailey, V. ; Lloyd, A. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-7018c4eb07761f38a0a2881c67bd57e638bad11710094a203f0b5e92f532cad63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Chromatography, Liquid - methods</topic><topic>dietary supplements</topic><topic>Dietary Supplements - analysis</topic><topic>Female</topic><topic>Glycine max - chemistry</topic><topic>Humans</topic><topic>isoflavone intake</topic><topic>Isoflavones - administration & dosage</topic><topic>Isoflavones - analysis</topic><topic>Isoflavones - chemistry</topic><topic>menopause</topic><topic>Menopause - drug effects</topic><topic>Phytoestrogens</topic><topic>Phytoestrogens - administration & dosage</topic><topic>Phytoestrogens - analysis</topic><topic>Phytoestrogens - chemistry</topic><topic>Reference Standards</topic><topic>soya, red clover</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Trifolium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clarke, D. B.</creatorcontrib><creatorcontrib>Bailey, V.</creatorcontrib><creatorcontrib>Lloyd, A. S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clarke, D. B.</au><au>Bailey, V.</au><au>Lloyd, A. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of phytoestrogens in dietary supplements by LC-MS/MS</atitle><jtitle>Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment</jtitle><addtitle>Food Addit Contam Part A Chem Anal Control Expo Risk Assess</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>25</volume><issue>5</issue><spage>534</spage><epage>547</epage><pages>534-547</pages><issn>1944-0049</issn><eissn>1944-0057</eissn><abstract>Labelling data quantifying the exact content of individual phytoestrogen analytes in dietary supplements are generally poor. As these products are commonly used in the management of menopause symptoms, any clinical benefits would be dependent on the exact dosage of isoflavones received. Well-established extraction procedures and updated isotope dilution mass spectrometry liquid chromatography coupled with tandem mass spectrometry detection (LC-MS/MS) have been used to accurately quantify the concentrations of ten common isoflavones in 35 dietary supplement samples on sale in the UK, Canada and Italy. Concentration-specific ionization suppression is described for biochanin A and formononetin. All supplements contained phytoestrogens. The soya isoflavones (genistein, daidzein, glycitein) were present in all products and the majority also contained the red clover isoflavones (biochanin A, formononetin) and some the Kudzu isoflavones (daidzein, puerarin). The content of total isoflavones per dose ranged from <1 to 53 mg. Trace amounts of coumestrol were found in six products. Other less common analytes, the prenylnaringenins (6-prenylnaringenin, 8-prenylnaringenin, 6,8-diprenylnaringenin) were not found in any of the products. Only 14 of 35 supplements were found to deliver more than or equal to 40 mg day
−1
of aglycone isoflavones, a consensus dose value recognized as delivering therapeutic benefit. Eleven did not match label claims. Six delivered less than 10 mg day
−1
of isoflavones. There has been little improvement in the overall quality of industry labelling in the five years since this was last investigated. Consequently, the public, retailers and healthcare professionals should consider using standardized isoflavone supplements, which are supported by analytical measurements.</abstract><cop>England</cop><pub>Taylor & Francis Group</pub><pmid>18478479</pmid><doi>10.1080/02652030701658340</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1944-0049 |
| ispartof | Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 2008-05, Vol.25 (5), p.534-547 |
| issn | 1944-0049 1944-0057 |
| language | eng |
| recordid | cdi_pubmed_primary_18478479 |
| source | Taylor and Francis:Jisc Collections:Taylor and Francis Read and Publish Agreement 2024-2025:Science and Technology Collection (Reading list) |
| subjects | Chromatography, Liquid - methods dietary supplements Dietary Supplements - analysis Female Glycine max - chemistry Humans isoflavone intake Isoflavones - administration & dosage Isoflavones - analysis Isoflavones - chemistry menopause Menopause - drug effects Phytoestrogens Phytoestrogens - administration & dosage Phytoestrogens - analysis Phytoestrogens - chemistry Reference Standards soya, red clover Tandem Mass Spectrometry - methods Trifolium - chemistry |
| title | Determination of phytoestrogens in dietary supplements by LC-MS/MS |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-09-21T12%3A40%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Determination%20of%20phytoestrogens%20in%20dietary%20supplements%20by%20LC-MS/MS&rft.jtitle=Food%20additives%20&%20contaminants.%20Part%20A,%20Chemistry,%20analysis,%20control,%20exposure%20&%20risk%20assessment&rft.au=Clarke,%20D.%20B.&rft.date=2008-05-01&rft.volume=25&rft.issue=5&rft.spage=534&rft.epage=547&rft.pages=534-547&rft.issn=1944-0049&rft.eissn=1944-0057&rft_id=info:doi/10.1080/02652030701658340&rft_dat=%3Cpubmed_cross%3E18478479%3C/pubmed_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c562t-7018c4eb07761f38a0a2881c67bd57e638bad11710094a203f0b5e92f532cad63%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/18478479&rfr_iscdi=true |