Determination of phytoestrogens in dietary supplements by LC-MS/MS

Labelling data quantifying the exact content of individual phytoestrogen analytes in dietary supplements are generally poor. As these products are commonly used in the management of menopause symptoms, any clinical benefits would be dependent on the exact dosage of isoflavones received. Well-establi...

Full description

Saved in:
Bibliographic Details
Published in:Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Chemistry, analysis, control, exposure & risk assessment, 2008-05, Vol.25 (5), p.534-547
Main Authors: Clarke, D. B., Bailey, V., Lloyd, A. S.
Format: Article
Language:English
Subjects:
Chromatography, Liquid - methods
dietary supplements
Dietary Supplements - analysis
Female
Glycine max - chemistry
Humans
isoflavone intake
Isoflavones - administration & dosage
Isoflavones - analysis
Isoflavones - chemistry
menopause
Menopause - drug effects
Phytoestrogens
Phytoestrogens - administration & dosage
Phytoestrogens - analysis
Phytoestrogens - chemistry
Reference Standards
soya, red clover
Tandem Mass Spectrometry - methods
Trifolium - chemistry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Labelling data quantifying the exact content of individual phytoestrogen analytes in dietary supplements are generally poor. As these products are commonly used in the management of menopause symptoms, any clinical benefits would be dependent on the exact dosage of isoflavones received. Well-established extraction procedures and updated isotope dilution mass spectrometry liquid chromatography coupled with tandem mass spectrometry detection (LC-MS/MS) have been used to accurately quantify the concentrations of ten common isoflavones in 35 dietary supplement samples on sale in the UK, Canada and Italy. Concentration-specific ionization suppression is described for biochanin A and formononetin. All supplements contained phytoestrogens. The soya isoflavones (genistein, daidzein, glycitein) were present in all products and the majority also contained the red clover isoflavones (biochanin A, formononetin) and some the Kudzu isoflavones (daidzein, puerarin). The content of total isoflavones per dose ranged from
ISSN:1944-0049
1944-0057
DOI:10.1080/02652030701658340