Prophage Sequences Defining Hot Spots of Genome Variation in Salmonella enterica Serovar Typhimurium Can Be Used To Discriminate between Field Isolates

Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by microbiological and molecular techniques, including pulsed-field gel electrophoresis (PFGE) and plasmid profiling....

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Bibliographic Details
Published in:Journal of Clinical Microbiology 2007-08, Vol.45 (8), p.2590-2598
Main Authors: Cooke, Fiona J, Wain, John, Fookes, Maria, Ivens, Alasdair, Thomson, Nicholas, Brown, Derek J, Threlfall, E. John, Gunn, George, Foster, Geoffrey, Dougan, Gordon
Format: Article
Language:English
Subjects:
Animals
Bacterial Typing Techniques - methods
Bacteriology
Biological and medical sciences
Cattle
Cluster Analysis
Crows
DNA Fingerprinting
DNA, Bacterial - genetics
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
Genome, Bacterial - genetics
Genomic Islands - genetics
Genotype
Humans
Microbiology
Miscellaneous
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Prophages - genetics
Recombination, Genetic
Salmonella enterica
Salmonella Infections - microbiology
Salmonella Infections, Animal - microbiology
Salmonella Phages - genetics
Salmonella typhimurium - classification
Salmonella typhimurium - genetics
Salmonella typhimurium - isolation & purification
Salmonella typhimurium - virology
Swine
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Summary:Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by microbiological and molecular techniques, including pulsed-field gel electrophoresis (PFGE) and plasmid profiling. PFGE identified 10 profiles that clustered by phage type and antibiotic resistance pattern with human and animal isolates distributed among different PFGE profiles. Genomic DNA was purified from 23 representative strains and hybridized to the composite Salmonella DNA microarray, and specific genomic regions that exhibited significant variation between isolates were identified. Bioinformatic analysis showed that variable regions of DNA were associated with prophage-like elements. Subsequently, simple multiplex PCR assays were designed on the basis of these variable regions that could be used to discriminate between S. enterica serovar Typhimurium isolates from the same geographical region. These multiplex PCR assays, based on prophage-like elements and Salmonella genomic island 1, provide a simple method for identifying new variants of S. enterica serovar Typhimurium in the field.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.00729-07