Autosomal STR Frequencies in Afghanistan Population

Buccal cells were collected by buccal brushes (Sterile Omni Swab, Whatman International Ltd.) from healthy, random Afghanian individuals. DNA was extracted by Chelex method (1). DNA samples were quantified using AmpFℓSTR® Quantifiler (Ap plied Biosystems, Foster City, CA) by Real time 7000 (Applied...

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Bibliographic Details
Published in:	Journal of forensic sciences 2005-11, Vol.50 (6), p.JFS2005237-3
Main Authors:	Berti, A, Barni, F, Virgili, A, Iacovacci, G, Franchi, C, Rapone, C, Di Carlo, A, Oddo, CM, Lago, G
Format:	Article
Language:	eng
Subjects:	Afghanistan DNA Fingerprinting Gene Frequency Genetics, Population Humans Polymerase Chain Reaction Tandem Repeat Sequences
Online Access:	Get full text
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Summary:	Buccal cells were collected by buccal brushes (Sterile Omni Swab, Whatman International Ltd.) from healthy, random Afghanian individuals. DNA was extracted by Chelex method (1). DNA samples were quantified using AmpFℓSTR® Quantifiler (Ap plied Biosystems, Foster City, CA) by Real time 7000 (Applied Biosystems, Foster City, CA), one nanogram of the samples obtained from the previous phase was amplified by GeneAmp® PCR System 9600 (Applied Biosystems, Foster City, CA). Simultaneous amplifications of 16 STRs loci (multiplexed PCR) were performed by using AmpFℓSTR® Identifiler™ (Applied Biosystems, Foster City, CA) according to the user manual recommendations (2).
ISSN:	0022-1198 1556-4029