Relationship between Interleukin-12-expressing Cells and Antigen-presenting Cells in Patients with Colorectal Cancer

Background: Interleukin-12 (IL-12) is a powerful cytokine that plays an important role in cell-mediated immunity. Although IL-12 is produced by antigen-presenting cells (APCs), the relationship between IL-12 expression and APCs in colorectal cancer tissue remains unknown. Patients and Methods: Immun...

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Bibliographic Details
Published in:Anticancer research 2005-09, Vol.25 (5), p.3541-3546
Main Authors: INOUE, Yuzuru, NAKAYAMA, Yoshifumi, ITOH, Hideaki, MINAGAWA, Noritaka, KATSUKI, Takefumi, NAGASHIMA, Nobuo, MATSUMOTO, Kentaro, SHIBAO, Kazunori, TSURUDOME, Yosuke, HIRATA, Keiji, NAGATA, Naoki
Format: Article
Language:English
Subjects:
Adult
Aged
Antigen-Presenting Cells - immunology
Antigen-Presenting Cells - metabolism
Antigens, CD - biosynthesis
Antigens, CD - immunology
Antigens, Differentiation, Myelomonocytic - biosynthesis
Antigens, Differentiation, Myelomonocytic - immunology
Biological and medical sciences
CD83 Antigen
Colorectal Neoplasms - immunology
Colorectal Neoplasms - pathology
Dendritic Cells - immunology
Dendritic Cells - metabolism
Female
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Immunoglobulins - biosynthesis
Immunoglobulins - immunology
Immunohistochemistry
Interleukin-12 - biosynthesis
Interleukin-12 - immunology
Interleukin-12 - metabolism
Macrophages - immunology
Macrophages - metabolism
Male
Medical sciences
Membrane Glycoproteins - biosynthesis
Membrane Glycoproteins - immunology
Middle Aged
Neoplasm Staging
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:Background: Interleukin-12 (IL-12) is a powerful cytokine that plays an important role in cell-mediated immunity. Although IL-12 is produced by antigen-presenting cells (APCs), the relationship between IL-12 expression and APCs in colorectal cancer tissue remains unknown. Patients and Methods: Immunohistochemical detection of APCs and IL-12 was performed in 22 colorectal cancer specimens. CD83 and CD68 were used for the markers of mature dendritic cells (DCs) and macrophages, respectively. Double staining with CD83 or CD68 and IL-12 was also performed to detect IL-12-secreting cells. CD83-, CD68- and IL-12-positive-cell densities, clinicopathological factors and survival were analyzed. Results: CD83-, CD68- or IL-12-positive-cells were stained in the tumor stroma. Double-stained CD83/IL-12- or CD68/IL-12-positive-cells were also detected in the same area. The CD83-positive-cell density was significantly higher in patients with a high IL-12-positive-cell density than those with a low IL-12-positive-cell density. The CD83-positive-cell density was significantly lower in patients with T3-T4 depth of invasion, lymph node metastasis or tumors more advanced than stage II. The IL-12-positive-cell density tended to be lower in patients with T3-T4 depth of invasion or venous invasion. Patients with high CD83- or IL-12-positive-cell density in their cancer specimens showed significantly better prognosis. Conclusion: This study provides new information on the significance of mature DCs, macrophages and IL-12-secreting cells in the local environment of colorectal cancer. Survival in patients with colorectal cancer was reflected by mature DCs and/or IL-12-positive-cell density.
ISSN:0250-7005
1791-7530