Memory-Influencing Intra-Basolateral Amygdala Drug Infusions Modulate Expression of Arc Protein in the Hippocampus

Activation of β-adrenoceptors in the basolateral complex of the amygdala (BLA) modulates memory storage processes and longterm potentiation in downstream targets of BLA efferents, including the hippocampus. Here, we show that this activation also increases hippocampal levels of activity-regulated cy...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-07, Vol.102 (30), p.10718-10723
Main Authors: McIntyre, Christa K., Miyashita, Teiko, Setlow, Barry, Marjon, Kristopher D., Steward, Oswald, Guzowski, John F., McGaugh, James L.
Format: Article
Language:English
Subjects:
Adrenergic beta-Agonists - pharmacology
Amygdala
Amygdala - metabolism
Analysis of Variance
Animals
Apoptosis Regulatory Proteins - genetics
Apoptosis Regulatory Proteins - metabolism
Avoidance Learning - drug effects
Behavioral neuroscience
Biological Sciences
Cerebral hemispheres
Clenbuterol - pharmacology
Drug therapy
Gene expression
Gene Expression Regulation - drug effects
Hemispheres
Hippocampus
Hippocampus - metabolism
Immunoblotting
In Situ Hybridization, Fluorescence
Influence
Lidocaine - pharmacology
Long term potentiation
Male
Memory
Memory - drug effects
Messenger RNA
Muscle Proteins - genetics
Muscle Proteins - metabolism
Neurology
Oligodeoxyribonucleotides, Antisense - pharmacology
Protein synthesis
Proteins
Rats
Rats, Sprague-Dawley
Vehicles
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Summary:Activation of β-adrenoceptors in the basolateral complex of the amygdala (BLA) modulates memory storage processes and longterm potentiation in downstream targets of BLA efferents, including the hippocampus. Here, we show that this activation also increases hippocampal levels of activity-regulated cytoskeletal protein (Arc), an immediate-early gene (also termed Arg 3.1) implicated in hippocampal synaptic plasticity and memory consolidation processes. Infusions of the β-adrenoreceptor agonist, clenbuterol, into the BLA immediately after training on an inhibitory avoidance task enhanced memory tested 48 h later. The same dose of clenbuterol significantly increased Arc protein levels in the dorsal hippocampus. Additionally, posttraining intra-BLA infusions of a memory-impairing dose of lidocaine significantly reduced Arc protein levels in the dorsal hippocampus. Increases in Arc protein levels were not accompanied by increases in Arc mRNA, suggesting that amygdala modulation of Arc protein and synaptic plasticity in efferent brain regions occurs at a posttranscriptional level. Finally, infusions of Arc antisense oligodeoxynucleotides into the dorsal hippocampus impaired performance of an inhibitory avoidance task, indicating that the changes in Arc protein expression are related to the observed changes in memory performance.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0504436102