LUX ARRHYTHMO encodes a Myb domain protein essential for circadian rhythms

In higher plants, the circadian clock orchestrates fundamental processes such as light signaling and the transition to flowering. We isolated mutants of the circadian clock from an Arabidopsis thaliana mutagenized reporter line by screening for seedlings with long hypocotyl phenotypes and subsequent...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2005-07, Vol.102 (29), p.10387-10392
Main Authors: Hazen, S.P, Schultz, T.F, Pruneda-Paz, J.L, Borevitz, J.O, Ecker, J.R, Kay, S.A
Format: Article
Language:English
Subjects:
Alleles
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
binding sites
Biological Clocks - genetics
Biological Sciences
Botany
Chromosome Mapping
Circadian rhythm
Circadian Rhythm - genetics
Control loops
DNA Primers
DNA-Binding Proteins - metabolism
Electrophoretic Mobility Shift Assay
flowering
gene expression
gene expression regulation
Gene Expression Regulation, Plant - genetics
genes
Genetic mutation
genetic techniques and protocols
Genetics
Hypocotyls
Light
LUX promoter
mutants
mutation
oligonucleotide array mapping
Oligonucleotide Array Sequence Analysis
Phenotypes
Photoperiod
Plant cells
Plants
promoter regions
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Seedlings
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:In higher plants, the circadian clock orchestrates fundamental processes such as light signaling and the transition to flowering. We isolated mutants of the circadian clock from an Arabidopsis thaliana mutagenized reporter line by screening for seedlings with long hypocotyl phenotypes and subsequently assaying for abnormal clock-regulated CAB2::LUC expression. This screen identified five mutant alleles of a clock gene, LUX ARRHYTHMO (LUX), that significantly affect amplitude and robustness of rhythms in both constant white light and dark conditions. In addition, the transition from vegetative to floral development is accelerated and hypocotyl elongation is accentuated in these mutants under light:dark cycles. We genetically mapped the mutations by bulk segregant analysis with high-density oligonucleotide array genotyping to a small putative Myb transcription factor related to other clock components and response regulators in Arabidopsis. The negative arm of the Arabidopsis circadian clock, CIRCADIAN CLOCK ASSOCIATED (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), is repressed in the lux mutants, whereas TIMING OF CAB2 EXPRESSION (TOC1) is activated. We demonstrate that CCA1 and LHY bind to the evening element motif in the LUX promoter, which strongly suggests that these proteins repress LUX expression, as they do TOC1. The data are also consistent with LUX being necessary for activation of CCA1 and LHY expression.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0503029102