Genetically Engineered Wine Yeast Produces a High Concentration of L-Lactic Acid of Extremely High Optical Purity

For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine L-lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, L-lactate production of this recombinant reached 122 g/liter and the opti...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2005-05, Vol.71 (5), p.2789-2792
Main Authors: Saitoh, Satoshi, Ishida, Nobuhiro, Onishi, Toru, Tokuhiro, Kenro, Nagamori, Eiji, Kitamoto, Katsuhiko, Takahashi, Haruo
Format: Article
Language:English
Subjects:
Biological and medical sciences
biosynthesis
Biotechnology
cattle
culture media
enantiomers
Fermentation
Food science
Fundamental and applied biological sciences. Psychology
gene dosage
gene expression
Genes
Genetic Engineering
genetically engineered microorganisms
L-Lactate Dehydrogenase - genetics
lactate dehydrogenase
lactic acid
Lactic Acid - biosynthesis
LDH gene
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microbiology
Physiology and Biotechnology
Plasmids
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
sugarcane juice
transgenes
Wine
wine yeasts
Yeast
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Summary:For mass production of lactic acid, we newly constructed a transgenic wine yeast strain that included six copies of the bovine L-lactate dehydrogenase gene on the genome. On fermentation in inexpensive cane juice-based medium, L-lactate production of this recombinant reached 122 g/liter and the optical purity was 99.9% or higher.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.71.5.2789-2792.2005