Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garcinia subelliptica

The tyrosinase inhibitory activity of methanol extracts of the leaves of 39 plant species growing on the seashore of Iriomote island (Okinawa. Japan) was investigated. The extracts of Hibiscus tiliaceus, Carex pumila. and Garcinia subelliptica showed potent activity among them. The inhibitors in the...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2005-01, Vol.69 (1), p.197-201
Main Authors: Masuda, T. (Tokushima Univ. (Japan)), Yamashita, D, Takeda, Y, Yonemori, S
Format: Article
Language:English
Subjects:
biflavonoid
Biological and medical sciences
Carex pumila
COASTS
ENZYME INHIBITORS
EXTRACTS
Flavanones - isolation & purification
Flavanones - pharmacology
Flavones - isolation & purification
Flavones - pharmacology
FLAVONOIDS
Fundamental and applied biological sciences. Psychology
GARCINIA
Garcinia - chemistry
Garcinia subelliptica
HIBISCUS TILIACEUS
IDENTIFICATION
Marine
Molecular Structure
Monophenol Monooxygenase - antagonists & inhibitors
OXIDOREDUCTASES
Plant Extracts - pharmacology
seashore plant
SELECTION
tyrosinase inhibitor
TYROSINE
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Summary:The tyrosinase inhibitory activity of methanol extracts of the leaves of 39 plant species growing on the seashore of Iriomote island (Okinawa. Japan) was investigated. The extracts of Hibiscus tiliaceus, Carex pumila. and Garcinia subelliptica showed potent activity among them. The inhibitors in the extract of Garcinia subelliptica were purified by assay-guided fractionation to give two biflavonoids, These were known compounds (2R,3S-5,7,4 min,5 secN,7 sec,3''',4'''-heptahydroxy tlavanone[3-8 sec] fiavone and 5,7,4 min,5 sec,7 sec,3''',4'''-heptahydroxy[3-8 sec] biflavanone), although their strong inhibitory activity toward tyrosinase is revealed for the first time in this work. One of these biflavonoids (2R,3S-5,7,4 min.5 secN,7 secN,3''', 4''',heptahydroxy flavanone[3-8 sec] flavone) showed much stronger activity (IC sub(50) 2.5 MicroM) than that of kojic acid (IC sub(50) 9.1 MicroM) when L-tyrosine was used as the substrate.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.69.197