Molecular cloning of a genomic DNA encoding yam class IV chitinase

Genomic DNA for a class IV chitinase was cloned from yam (Dioscorea opposita Thunb) leaves and sequenced. The deduced amino acid sequence shows 50 to 59% identity to class IV chitinases from other plants. The yam chitinase, however, has an additional sequence of 8 amino acids (a C-terminal extension...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-07, Vol.68 (7), p.1508-1517
Main Authors: Mitsunaga, T. (Yamaguchi Univ. (Japan). Faculty of Agriculture), Iwase, M, Ubhayasekera, W, Mowbray, S.L, Koga, S
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Catalysis
CHITINASE
Chitinases - chemistry
Chitinases - classification
Chitinases - genetics
class II chitinase
class IV chitinase
CLASSIFICATION
Cloning, Molecular
Dioscorea
Dioscorea - enzymology
Dioscorea - genetics
ELECTROPHORESIS
extension polymerase chain reaction (PCR) method
Fundamental and applied biological sciences. Psychology
FUNGICIDES
Genes, Plant
GENOMES
Hordeum vulgare
Models, Molecular
MOLECULAR CLONING
Molecular Sequence Data
PATHOGENS
PCR
Plant Leaves - enzymology
Plant Leaves - genetics
Protein Structure, Tertiary
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
yam (Dioscorea opposita Thunb)
YAMS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genomic DNA for a class IV chitinase was cloned from yam (Dioscorea opposita Thunb) leaves and sequenced. The deduced amino acid sequence shows 50 to 59% identity to class IV chitinases from other plants. The yam chitinase, however, has an additional sequence of 8 amino acids (a C-terminal extension) following the cysteine that was reported as the last amino acid for other class IV chitinases; this extension is perhaps involved in subcelluiar localization. A homology model based on the structure of a class n chitinase from barley was used as an aid to interpreting the available data. The analysis suggests that the class IV enzyme recognizes an even shorter segment of the substrate than class I or II enzymes. This observation might help to explain why class IV enzymes are better suited to attack against pathogen cell walls.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.68.1508