Human biomonitoring of pyrethrum and pyrethroid insecticides used indoors:: Determination of the metabolites E- cis/ trans-chrysanthemumdicarboxylic acid in human urine by gas chromatography-mass spectrometry with negative chemical ionization

This work describes a gas chromatographic-mass spectrometric method employing negative chemical ionization (NCI) for the determination of E- cis/ trans-chrysanthemumdicarboxylic acid (CDCA) in human urine used as a biomarker for the exposure to pyrethrum and/or certain pyrethroids in insecticide for...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-10, Vol.795 (2), p.195-207
Main Authors: Elflein, L, Berger-Preiss, E, Preiss, A, Elend, M, Levsen, K, Wünsch, G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calibration
Chrysanthemumdicarboxylic acid
Derivatization, GC
Environmental Monitoring
Gas Chromatography-Mass Spectrometry - methods
Humans
Insecticides - analysis
Magnetic Resonance Spectroscopy
Medical sciences
Pesticides, fertilizers and other agrochemicals toxicology
Pyrethrins - analysis
Pyrethrins - urine
Pyrethroid insecticides
Pyrethrum
Reproducibility of Results
Toxicology
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Summary:This work describes a gas chromatographic-mass spectrometric method employing negative chemical ionization (NCI) for the determination of E- cis/ trans-chrysanthemumdicarboxylic acid (CDCA) in human urine used as a biomarker for the exposure to pyrethrum and/or certain pyrethroids in insecticide formulations applied indoors. Mixed-mode solid phase extraction was utilized for sample cleanup. Extraction recoveries ranged from 92 to 104% (2–9% R.S.D.). The acids were esterified with 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) allowing both their gas chromatographic separation and their sensitive mass spectrometric detection under NCI conditions. Detection limits of ca. 0.05 μg/l urine were achieved.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(03)00566-X