A 1-Mb Resolution Radiation Hybrid Map of the Canine Genome

The purebred dog population consists of >300 partially inbred genetic isolates or breeds. Restriction of gene flow between breeds, together with strong selection for traits, has led to the establishment of a unique resource for dissecting the genetic basis of simple and complex mammalian traits....

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2003-04, Vol.100 (9), p.5296-5301
Main Authors: Guyon, Richard, Lorentzen, Travis D., Hitte, Christophe, Kim, Lisa, Cadieu, Edouard, Parker, Heidi G., Quignon, Pascale, Lowe, Jennifer K., Renier, Corinne, Gelfenbeyn, Boris, Vignaux, Françoise, DeFrance, Hawkins B., Gloux, Stephanie, Mahairas, Gregory G., André, Catherine, Galibert, Francis, Ostrander, Elaine A.
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Canines
Chromosomes
Chromosomes, Artificial, Bacterial
Cricetinae
Dogs
Expressed Sequence Tags
Genes
Genome
Genomes
Genomics
Humans
Hybrid Cells - radiation effects
Microsatellite Repeats
Polymorphism, Single Nucleotide
Radiation
Radiation hybrid mapping
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Summary:The purebred dog population consists of >300 partially inbred genetic isolates or breeds. Restriction of gene flow between breeds, together with strong selection for traits, has led to the establishment of a unique resource for dissecting the genetic basis of simple and complex mammalian traits. Toward this end, we present a comprehensive radiation hybrid map of the canine genome composed of 3,270 markers including 1,596 microsatellite-based markers, 900 cloned gene sequences and ESTs, 668 canine-specific bacterial artificial chromosome (BAC) ends, and 106 sequence-tagged sites. The map was constructed by using the RHDF5000-2 whole-genome radiation hybrid panel and computed by using Multimap and TSP/Concorde. The 3,270 markers map to 3,021 unique positions and define an average intermarker distance corresponding to 1 Mb. We also define a minimal screening set of 325 highly informative well spaced markers, to be used in the initiation of genome-wide scans. The well defined synteny between the dog and human genomes, established in part as a function of this work by the identification of 85 conserved fragments, will allow follow-up of initial findings of linkage by selection of candidate genes from the human genome sequence. This work continues to define the canine system as the method of choice in the pursuit of the genes causing mammalian variation and disease.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0831002100