Detection of Secreted Peptides by Using Hypothesis-Driven Multistage Mass Spectrometry

A method is presented for the rapid detection and characterization of trace amounts of peptides secreted from microorganisms, including pheromones, virulence factors, and quorum-sensing peptides. The procedure, based on targeted multistage MS, uses a novel matrix-assisted laser desorption/ionization...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2003-03, Vol.100 (5), p.2795-2800
Main Authors: Kalkum, Markus, Lyon, Gholson J., Chait, Brian T.
Format: Article
Language:English
Subjects:
Biological Sciences
Diploidy
Haploidy
Ions
Lactones
Mass spectra
Mass Spectrometry
Mass spectroscopy
Mating Factor
Peptides
Peptides - chemistry
Peptides - metabolism
Proteins
Saccharomyces cerevisiae
Scientific imaging
Signal Transduction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Staphylococcus
Staphylococcus - metabolism
Staphylococcus aureus
Staphylococcus aureus - metabolism
Staphylococcus intermedius
T lymphocytes
Virulence
Yeasts
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Summary:A method is presented for the rapid detection and characterization of trace amounts of peptides secreted from microorganisms, including pheromones, virulence factors, and quorum-sensing peptides. The procedure, based on targeted multistage MS, uses a novel matrix-assisted laser desorption/ionization-ion trap mass spectrometer to overcome limitations of current MS methods (limited dynamic range, signal suppression effects, and chemical noise) that impair observation of low abundance peptides from complex biological matrixes. Here, secreted peptides that are hypothesized to be present in the supernatant, but that may not be sufficiently abundant to be observed in single-stage mass spectra, are subjected to multistage MS. Highly specific fragmentation signatures enable unambiguous identification of the peptides of interest and differentiation of the signals from the background. As examples, we demonstrate the rapid (
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0436605100