Cause and effect relationship between myocardial mast cell number and matrix metalloproteinase activity

Department of Anatomy, Physiology and Pharmacology, Auburn University, Auburn, Alabama 36849-5517 The objectives of this study were to investigate the temporal response of left ventricular (LV) matrix metalloproteinase (MMP) activity and collagen volume fraction (CVF) induced by an aortocaval fistul...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology. Heart and circulatory physiology 2002-08, Vol.283 (2), p.H518-H525
Main Authors: Brower, Gregory L, Chancey, Amanda L, Thanigaraj, Srihari, Matsubara, Beatriz B, Janicki, Joseph S
Format: Article
Language:English
Subjects:
Animals
Aortic Diseases - enzymology
Arteriovenous Fistula - enzymology
Cell Count
Cell Degranulation
Collagen - metabolism
Extracellular Space - metabolism
Male
Mast Cells - cytology
Mast Cells - physiology
Matrix Metalloproteinases - metabolism
Myocardium - cytology
Myocardium - enzymology
Myocardium - metabolism
Rats
Rats, Sprague-Dawley
Venae Cavae - abnormalities
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Department of Anatomy, Physiology and Pharmacology, Auburn University, Auburn, Alabama 36849-5517 The objectives of this study were to investigate the temporal response of left ventricular (LV) matrix metalloproteinase (MMP) activity and collagen volume fraction (CVF) induced by an aortocaval fistula and the role of cardiac mast cells in regulating MMP activity. LV tissue was analyzed for MMP activity, CVF, and mast cell number in rats euthanized at 0.5, 1, 2, 3, 5, 14, 21, 35, and 56 days. Additional rats treated with the mast cell membrane-stabilizing drug cromolyn sodium were euthanized 1, 2, and 3 days postfistula. Marked increases in MMP activity occurred rapidly and remained significantly elevated for 5 days before returning toward normal. A significant decrease in CVF occurred by day 5 , but thereafter CVF rebounded to normal or above normal values. The number of myocardial mast cells also significantly increased postfistula, and there was a close association between mast cell density and MMP activity. Cromolyn treatment prevented the increase in mast cell number and MMP activity. Thus it is concluded that cardiac mast cells play a major role in the regulation of MMP activity. myocardial collagen; gelatinase; cromolyn sodium; aortocaval fistula; extracellular matrix remodeling
ISSN:0363-6135
1522-1539
DOI:10.1152/ajpheart.00218.2000