Direct measurement of the lumped constant for 2-deoxy-[1-14C]glucose in vivo in human skeletal muscle

1  Division of Diabetology, Department of Medicine, University of Helsinki, FIN-00029 HUCH, Helsinki, Finland; 2  Department of Electronics and Informatics, University of Padova, 35131 Padua, Italy; and 3  The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 The lum...

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Published in:American journal of physiology: endocrinology and metabolism 2000-07, Vol.279 (1), p.E228-E233
Main Authors: Utriainen, Tapio, Lovisatti, Stefania, Makimattila, Sari, Bertoldo, Alessandra, Weintraub, Susan, DeFronzo, Ralph, Cobelli, Claudio, Yki-Jarvinen, Hannele
Format: Article
Language:English
Subjects:
Adult
Blood Glucose - analysis
Carbon Radioisotopes
Deoxyglucose - pharmacokinetics
Eating - physiology
Forearm
Glucose - pharmacokinetics
Glucose Clamp Technique
Humans
Insulin - blood
Male
Mannitol - blood
Models, Biological
Muscle, Skeletal - blood supply
Muscle, Skeletal - metabolism
Osmolar Concentration
Reference Values
Regional Blood Flow
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Summary:1  Division of Diabetology, Department of Medicine, University of Helsinki, FIN-00029 HUCH, Helsinki, Finland; 2  Department of Electronics and Informatics, University of Padova, 35131 Padua, Italy; and 3  The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229 The lumped constant (LC) is used to convert the clearance rate of 2-deoxy- D -glucose (2-DG CR ) to that of glucose (Glc CR ). There are currently no data to validate the widely used assumption of an LC of 1.0 for human skeletal muscle. We determined the LC for 2-deoxy-[1- 14 C]glucose (2-DG) in 18 normal male subjects (age, 29 ± 2 yr; body mass index, 24.8 ± 0.8 kg/m 2 ) after an overnight fast and during physiological (1 mU · kg 1 · min 1 insulin infusion for 180 min) and supraphysiological (5 mU ·   kg 1 · min 1 insulin infusion for 180 min) hyperinsulinemic conditions. Normoglycemia was maintained with the euglycemic clamp technique. The LC was measured directly with the use of a novel triple tracer-based method. [3- 3 H]glucose, 2-[1- 14 C]DG, and [ 12 C]mannitol (Man) were injected as a bolus into the brachial artery. The concentrations of [3- 3 H]glucose and 2-[1- 14 C]DG (dpm/ml plasma) and of Man (µmol/l) were determined in 50   blood samples withdrawn from the ipsilateral deep forearm vein over 15 min after the bolus injection. The LC was calculated by a formula involving blood flow calculated from Man and the Glc CR and 2-DG CR . The LC averaged 1.26 ± 0.08 (range 1.06-1.43), 1.15 ± 0.05 (0.99-1.39), and 1.18 ± 0.05 (0.97-1.37) under fasting conditions and during the 1 and 5 mU · kg 1 · min 1 insulin infusions (not significant between the different insulin concentrations, mean LC = 1.2,  P  
ISSN:0193-1849
1522-1555
DOI:10.1152/ajpendo.2000.279.1.e228