Improved Recovery of Mycobacterium tuberculosis from Pleural Aspirates: Bedside Inoculation, Heparinized Containers and Liquid Culture Media

The effects of bedside inoculation, heparinized containers and liquid culture media on the recovery of Mycobacterium tuberculosis from pleural aspirates were evaluated in this study. Of 155 patients, 63 were diagnosed to have pleural effusion tuberculous in origin. The overall recovery of M. tubercu...

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Bibliographic Details
Published in:Scandinavian journal of infectious diseases 1999, Vol.31 (5), p.485-487
Main Authors: CHENG, A. F. B, TAI, V. H. B, LI, M. S. K, CHAN, C. H. S, WONG, C. F, YEW, W. W, HUI, M, CHAN, C. Y, LEE, J. C. K
Format: Article
Language:English
Subjects:
Anticoagulants
Bacterial diseases
Bacterial diseases of the respiratory system
Bacteriological Techniques
Biological and medical sciences
Culture Media
Heparin
Human bacterial diseases
Humans
Infectious diseases
Medical sciences
Mycobacterium tuberculosis
Mycobacterium tuberculosis - isolation & purification
Specimen Handling - methods
Tuberculosis, Pleural - microbiology
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Summary:The effects of bedside inoculation, heparinized containers and liquid culture media on the recovery of Mycobacterium tuberculosis from pleural aspirates were evaluated in this study. Of 155 patients, 63 were diagnosed to have pleural effusion tuberculous in origin. The overall recovery of M. tuberculosis was 57.1%. Bedside inoculation of the specimens produced a significantly higher yield than laboratory inoculation using non-heparinized specimens. When the pleural aspirates were transported in heparinized containers, the recovery rate was comparable to that from bedside inoculation, but lower when non-heparinized containers were used. No significant difference was found in recovery rate between the two liquid media, but the rate was significantly higher with the use of liquid media than conventional solid media. Thus, bedside inoculation of pleural aspirates, use of heparinized containers for transport for delayed inoculation in the laboratory and use of liquid culture media are recommended.
ISSN:0036-5548
1651-1980
DOI:10.1080/00365549950164012