Improving HPLC Separation of Polyphenols

The in-depth study of polyphenols is hampered by their sheer complexity, combined with the inherent limitations of conventional high performance liquid chromatography (HPLC) separation methods. In this paper, the beneficial application of a number of recent developments in HPLC for polyphenol analys...

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Bibliographic Details
Published in:LC GC North America 2010-11, Vol.28 (11), p.978
Main Authors: DE VILLIERS, André, KALILI, Kathithileni M, MALAN, Mareta, ROODMAN, Jeanine
Format: Magazinearticle
Language:English
Subjects:
Analysis
Analytical chemistry
Anthocyanin
Biological and medical sciences
Chromatography
Experiments
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
General pharmacology
Medical sciences
Methods
Methods of analysis, processing and quality control, regulation, standards
Natural products
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Polymerization
Polyphenols
Temperature
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Summary:The in-depth study of polyphenols is hampered by their sheer complexity, combined with the inherent limitations of conventional high performance liquid chromatography (HPLC) separation methods. In this paper, the beneficial application of a number of recent developments in HPLC for polyphenol analysis is highlighted. Small particle-packed columns operated at elevated pressures and temperatures are used to improve the speed and resolving power of conventional HPLC methods. In addition, comprehensive two-dimensional LC combining hydrophilic interaction chromatography (HILIC) and reversed-phase LC is shown to be a powerful technique to increase the resolving power. Examples of the successful application of these technologies for improved analysis of procyanidins, flavonols, and anthocyanins in wine, tea, cocoa, and blueberry samples are presented. [PUBLICATION ABSTRACT]
ISSN:1527-5949
1939-1889