Adipose-Derived Stem-Cell Treatment of Skeletal Muscle Injury

BACKGROUND:The aim of the present study was to investigate whether adipose-derived stem cells could contribute to skeletal muscle-healing. METHODS:Adipose-derived stem cells of male rats were cultured and injected into the soleus muscles of female rats. Two and four weeks after injections, muscles w...

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Published in:	Journal of bone and joint surgery. American volume 2012-04, Vol.94 (7), p.609-617
Main Authors:	Peçanha, Ramon, Bagno, Luiza de Lima e Silva, Ribeiro, Marcelo Baldanza, Robottom Ferreira, Anna Beatriz, Moraes, Milton Ozório, Zapata-Sudo, Gisele, Kasai-Brunswick, Taís Hanae, Campos-de-Carvalho, Antônio Carlos, Goldenberg, Regina Coeli dos Santos, Saar Werneck-de-Castro, João Pedro
Format:	Article
Language:	eng
Subjects:	Adipocytes - transplantation Analysis of Variance Animals Biological and medical sciences Biopsy, Needle Disease Models, Animal Diseases of the osteoarticular system Female Graft Rejection Graft Survival Immunohistochemistry Male Medical sciences Muscle Contraction - physiology Muscle Strength - physiology Muscle, Skeletal - injuries Muscle, Skeletal - pathology Muscle, Skeletal - surgery Orthopedic surgery Random Allocation Rats Rats, Inbred Strains Real-Time Polymerase Chain Reaction - methods Regeneration - physiology Risk Assessment Sensitivity and Specificity Stem Cell Transplantation - methods Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Wounds and Injuries - pathology Wounds and Injuries - therapy
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Summary:	BACKGROUND:The aim of the present study was to investigate whether adipose-derived stem cells could contribute to skeletal muscle-healing. METHODS:Adipose-derived stem cells of male rats were cultured and injected into the soleus muscles of female rats. Two and four weeks after injections, muscles were tested for tetanic force (50 Hz). Histological analysis was performed to evaluate muscle collagen deposition and the number of centronucleated muscle fibers. In order to track donor cells, chimerism was detected with use of real-time polymerase chain reaction targeting the male sex-determining region Y (SRY) gene. RESULTS:Two weeks after cell injection, tetanus strength and the number of centronucleated regenerating myofibers, as well as the number of centronucleated regenerating myofibers, were higher in the treated group than they were in the control group (mean and standard error of the mean, 79.2 ± 5.0% versus 58.3 ± 8.1%, respectively [p < 0.05]; and 145 ± 36 versus 273 ± 18 per 10 myofibers, respectively [p < 0.05]). However, there were no significant differences at four weeks. Treatment did not decrease collagen deposition. Male gene was not detected in female host tissue at two and four weeks after engraftment by polymerase chain reaction analysis. CONCLUSIONS:Adipose-derived stem-cell therapy increased muscle repair and force at two weeks, but not four weeks, after injection, suggesting that adipose-derived stem-cell administration may accelerate muscle repair; however, the rapid disappearance of injected cells suggests a paracrine mechanism of action.
ISSN:	0021-9355 1535-1386