Urinary mRNA markers of epithelial-mesenchymal transition correlate with progression of diabetic nephropathy

Summary Objective  Epithelial–mesenchymal transition (EMT) plays an important role in the pathogenesis and progression of diabetic nephropathy (DN). Quantification of messenger RNA (mRNA) expression in urinary sediment is emerging as a noninvasive method of screening DN‐associated biomarkers. The ai...

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Bibliographic Details
Published in:Clinical endocrinology (Oxford) 2012-05, Vol.76 (5), p.657-664
Main Authors: Zheng, Min, Lv, Lin-Li, Cao, Yu-Han, Zhang, Jian-Dong, Wu, Min, Ma, Kun-Ling, Phillips, Aled O., Liu, Bi-Cheng
Format: Article
Language:English
Subjects:
Abundance
Actin
Actins - genetics
Adult
Aged
Albumin
Albuminuria - urine
Associated diseases and complications
Biological and medical sciences
biomarkers
Biomarkers - urine
Blood
Blood Urea Nitrogen
Creatinine
Diabetes mellitus
Diabetes. Impaired glucose tolerance
Diabetic Nephropathies - genetics
Diabetic Nephropathies - pathology
Diabetic Nephropathies - urine
Disease Progression
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Epidermal growth factor receptors
Epithelial-Mesenchymal Transition - genetics
Excretion
Female
Fibronectin
Fibronectins - genetics
Fundamental and applied biological sciences. Psychology
Gelatinase B
Gene Expression
Glomerular Filtration Rate
Humans
Kidneys
Male
Matrix Metalloproteinase 9 - genetics
Medical sciences
Middle Aged
Muscle, Smooth - metabolism
Muscles
Nephrology. Urinary tract diseases
Nephropathy
Nitrogen
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - urine
Sediments
Urea
Urinary system involvement in other diseases. Miscellaneous
Vertebrates: endocrinology
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Summary:Summary Objective  Epithelial–mesenchymal transition (EMT) plays an important role in the pathogenesis and progression of diabetic nephropathy (DN). Quantification of messenger RNA (mRNA) expression in urinary sediment is emerging as a noninvasive method of screening DN‐associated biomarkers. The aim of our study was to examine whether urinary mRNA profile of EMT‐associated genes may provide valuable clinical insight into the different stages of DN. Design and methods  Diabetic nephropathy patients (n = 44) and healthy controls (n = 12) were enrolled in this study. DN patients were divided into three groups according to the levels of estimated glomerular filtration rate (eGFR): Group A (eGFR > 60 ml/min per 1·73 m2, n = 27), Group B (45 
ISSN:0300-0664
1365-2265
DOI:10.1111/j.1365-2265.2011.04192.x