Biomineralization-based conversion of carbon dioxide to calcium carbonate using recombinant carbonic anhydrase

► Biomineralization-based conversion of CO2 to biominerals using recombinant carbonic anhydrase (CA) biocatalyst. ► Highly expressed recombinant α-type CA originating in Neisseria gonorrhoeae in Escherichia coli. ► Comparable CO2 hydration activity of recombinant N. gonorrhoeae CA to commercial bovi...

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Bibliographic Details
Published in:Chemosphere (Oxford) 2012-06, Vol.87 (10), p.1091-1096
Main Authors: Kim, Im Gyu, Jo, Byung Hoon, Kang, Dong Gyun, Kim, Chang Sup, Choi, Yoo Seong, Cha, Hyung Joon
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomineralization
Biotechnology
Blotting, Western
Calcium carbonate
Calcium Carbonate - analysis
Calcium Carbonate - metabolism
Carbon dioxide
Carbon Dioxide - analysis
Carbon Dioxide - metabolism
Carbon Sequestration
Carbonic anhydrase
Carbonic Anhydrases - economics
Carbonic Anhydrases - genetics
Carbonic Anhydrases - metabolism
Cattle
Climatology. Bioclimatology. Climate change
Cloning, Molecular
Conversion
Earth, ocean, space
Electrophoresis, Polyacrylamide Gel
Escherichia coli - enzymology
Escherichia coli - genetics
Exact sciences and technology
External geophysics
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Industrial applications and implications. Economical aspects
Industrial Microbiology - economics
Industrial Microbiology - methods
Meteorology
Neisseria gonorrhoeae - enzymology
Neisseria gonorrhoeae - genetics
Other applications
Recombinant Proteins - economics
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
X-Ray Diffraction
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Summary:► Biomineralization-based conversion of CO2 to biominerals using recombinant carbonic anhydrase (CA) biocatalyst. ► Highly expressed recombinant α-type CA originating in Neisseria gonorrhoeae in Escherichia coli. ► Comparable CO2 hydration activity of recombinant N. gonorrhoeae CA to commercial bovine CA. ► Significantly promoted formation of solid CaCO3 through the acceleration of CO2 hydration rate. Recently, as a mimic of the natural biomineralization process, the use of carbonic anhydrase (CA), which is an enzyme catalyzing fast reversible hydration of carbon dioxide to bicarbonate, has been suggested for biological conversion of CO2 to valuable chemicals. While purified bovine CA (BCA) has been used in previous studies, its practical utilization in CO2 conversion has been limited due to the expense of BCA preparation. In the present work, we investigated conversion of CO2 into calcium carbonate as a target carbonate mineral by using a more economical, recombinant CA. To our knowledge, this is the first report of the usage of recombinant CA for biological CO2 conversion. Recombinant α-type CA originating in Neisseria gonorrhoeae (NCA) was highly expressed as a soluble form in Escherichia coli. We found that purified recombinant NCA which showed comparable CO2 hydration activity to commercial BCA significantly promoted formation of solid CaCO3 through the acceleration of CO2 hydration rate, which is naturally slow. In addition, the rate of calcite crystal formation was also accelerated using recombinant NCA. Moreover, non-purified crude recombinant NCA also showed relatively significant ability. Therefore, recombinant CA could be an effective, economical biocatalyst in practical CO2 conversion system.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2012.02.003