genetic linkage map of durum wheat

A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Kor...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998-10, Vol.97 (5/6), p.721-728
Main Authors: Blanco, A, Bellomo, M.P, Cenci, A, De Giovanni, C, D'Ovidio, R, Iacono, E, Laddomada, B, Pagnotta, M.A, Porceddu, E, Sciancalepore, A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological markers
biomarkers
Chromosome mapping
Classical genetics, quantitative genetics, hybrids
Durum wheat
Fundamental and applied biological sciences. Psychology
Genetic aspects
genetic markers
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Genomics
glutenins
hybrids
Identification and classification
inbred lines
interspecific hybridization
isozymes
Linkage (Genetics)
linkage groups
loci
morphological markers
Plant genetics
plant morphology
plant proteins
Pteridophyta, spermatophyta
recombinant inbred lines
restriction fragment length polymorphism
seed storage proteins
Segregation
segregation distortion
Triticum aestivum
Triticum turgidum
Triticum turgidum subsp. dicoccoides
Vegetals
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Summary:A genetic linkage map of tetraploid wheat [Triticum turgidum (L.) Thell.] was constructed using segregation data from a population of 65 recombinant inbred lines (RILs) derived from a cross between the durum wheat cultivar Messapia and accession MG4343 of T. turgidum (L.) Thell. ssp dicoccoides (Korn.) Thell. A total of 259 loci were analysed, including 244 restriction fragment length polymorphisms (RFLPs), one PCR (polymerase chain reaction) marker (a sequence coding for a LMW (low-molecular-weight) glutenin subunit gene located at the Glu-B3 locus), seven biochemical (six seed-storage protein loci and one isozyme locus) and seven morphological markers. A total of 213 loci were mapped at a LOD greater than or equal to 3 on all 14 chromosomes of the A and B genomes. The total length of the map 15 1352 cM and the average distance between adjacent markers is 6.3 cM. Forty six loci could not be mapped at a LOD greater than or equal to 3. A fraction (18.6%) of the markers deviated significantly from the expected Mendelian ratios; clusters of loci showing distorted segregation were found on chromosomes 1B, 3AL, 4AL, 6AL and 7AL. The durum wheat map was compared with the published maps of bread wheat using several common RFLP markers and general features are discussed. The markers detected the known structural rearrangements involving chromosomes 4A, 5A and 7B as well as the translocation between 2B-6B, but not the deletion on 2BS. This map provides a useful tool for analysing and breeding economically important quantitative traits and for marker-assisted selection, as well as for studies of genome organisation in small grain cereal species.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050948