Isolation and characterization of microsatellites in trembling aspen (Populus tremuloides)

We have identified, isolated, and characterized microsatellite/simple sequence repeat (SSR) loci in trembling aspen (Populus tremuloides) by screening partial genomic libraries. We have also examined the compatibility and use of the P. tremuloides SSR primers to resolve microsatellites in other Popu...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998-05, Vol.96 (6/7), p.950-956
Main Authors: Dayanandan, S, Rajora, O.P, Bawa, K.S
Format: Article
Language:English
Subjects:
alleles
Animal populations
Aspen
Biological and medical sciences
Biological diversity
Classical genetics, quantitative genetics, hybrids
DNA sequencing
Fundamental and applied biological sciences. Psychology
gene frequency
Genetic aspects
Genetic diversity
Genetics of eukaryotes. Biological and molecular evolution
Genomic libraries
heterozygosity
loci
microsatellite repeats
Nucleotide sequencing
Physiological aspects
Plant genetics
Populus
Populus canadensis
Populus deltoides
Populus maximowiczii
Populus nigra
Populus tremuloides
Pteridophyta, spermatophyta
repetitive sequences
Vegetals
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Summary:We have identified, isolated, and characterized microsatellite/simple sequence repeat (SSR) loci in trembling aspen (Populus tremuloides) by screening partial genomic libraries. We have also examined the compatibility and use of the P. tremuloides SSR primers to resolve microsatellites in other Populus species. Fourteen microsatellites were identified from 1600 clones screened. The TC/AG microsatellites were the most abundant. A total of 29 alleles were detected in 36 P. tremuloides individuals at the four SSR loci (two each of di- and tri-nucleotide repeats) characterized. The number of alleles at the SSR loci ranged from 5 to 11, with an average of 7.25 alleles per locus, and the observed heterozygosity ranged from 0.19 to 0.82, with a mean of 0.46 per locus. Although the highest polymorphism was observed for a dinucleotide SSR locus, the trinucleotide SSR loci showed substantial polymorphism. There were 34 unique multilocus genotypes among the 36 P. tremuloides individuals examined, and 89% of the individuals had unique multilocus genotypes. Two pairs of SSR primers were successful in PCR, amplifying genomic DNA and resolving microsatellites of comparable size from Populus deltoides, P. nigra, P. x canadensis, and P. maximowiczii. The microsatellite DNA markers developed could be used for clonal fingerprinting, certification of controlled crosses, genome mapping, marker-assisted early selection, genetic diversity assessments, and conservation and sustainable management of poplar genetic resources.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050825