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Fast and reliable genotype validation using microsatellite markers in Arabidopsis thaliana
In this paper we show how rogue genotypes in the parental stocks or contaminants among the crossed progeny of Arabidopsis thaliana can be readily identified and excluded from the breeding process using microsatellite markers derived from a small quantity of intact leaf tissue which has been alkali-t...
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| Published in: | Theoretical and applied genetics 1999-03, Vol.98 (3/4), p.462-464 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c412t-4447120704fafe1b11d1b67b027adc2bc05492cb0815fbd056f6e9e6f8a00a263 |
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| container_end_page | 464 |
| container_issue | 3/4 |
| container_start_page | 462 |
| container_title | Theoretical and applied genetics |
| container_volume | 98 |
| creator | Virk, P.S Pooni, H.S Syed, N.H Kearsey, M.J |
| description | In this paper we show how rogue genotypes in the parental stocks or contaminants among the crossed progeny of Arabidopsis thaliana can be readily identified and excluded from the breeding process using microsatellite markers derived from a small quantity of intact leaf tissue which has been alkali-treated. This method is fast and cost effective as it does not require DNA extraction, is highly reliable, and is less damaging to small plants where only limited quantities of plant tissue are available. Furthermore, a large number of samples can be processed in 1 day, facilitating the identification process prior to selfing or crossing the plants. In addition, the procedure could potentially be automated since no centrifugation is required. |
| doi_str_mv | 10.1007/s001220051092 |
| format | article |
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This method is fast and cost effective as it does not require DNA extraction, is highly reliable, and is less damaging to small plants where only limited quantities of plant tissue are available. Furthermore, a large number of samples can be processed in 1 day, facilitating the identification process prior to selfing or crossing the plants. In addition, the procedure could potentially be automated since no centrifugation is required.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s001220051092</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>Arabidopsis thaliana ; Biological and medical sciences ; Chromosomes ; Classical genetics, quantitative genetics, hybrids ; crossing ; DNA ; extraction ; Fundamental and applied biological sciences. Psychology ; Genetic aspects ; genetic markers ; Genetics of eukaryotes. Biological and molecular evolution ; Genotype ; identification ; Identification and classification ; inbred lines ; leaves ; microsatellite repeats ; Microsatellites (Genetics) ; pedigree ; Plant genetics ; polymerase chain reaction ; Pteridophyta, spermatophyta ; Quality control ; recombinant inbred lines ; sodium hydroxide treatment ; Vegetals</subject><ispartof>Theoretical and applied genetics, 1999-03, Vol.98 (3/4), p.462-464</ispartof><rights>1999 INIST-CNRS</rights><rights>COPYRIGHT 1999 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-4447120704fafe1b11d1b67b027adc2bc05492cb0815fbd056f6e9e6f8a00a263</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,27957,27958</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1757125$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Virk, P.S</creatorcontrib><creatorcontrib>Pooni, H.S</creatorcontrib><creatorcontrib>Syed, N.H</creatorcontrib><creatorcontrib>Kearsey, M.J</creatorcontrib><title>Fast and reliable genotype validation using microsatellite markers in Arabidopsis thaliana</title><title>Theoretical and applied genetics</title><description>In this paper we show how rogue genotypes in the parental stocks or contaminants among the crossed progeny of Arabidopsis thaliana can be readily identified and excluded from the breeding process using microsatellite markers derived from a small quantity of intact leaf tissue which has been alkali-treated. 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Psychology</topic><topic>Genetic aspects</topic><topic>genetic markers</topic><topic>Genetics of eukaryotes. 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This method is fast and cost effective as it does not require DNA extraction, is highly reliable, and is less damaging to small plants where only limited quantities of plant tissue are available. Furthermore, a large number of samples can be processed in 1 day, facilitating the identification process prior to selfing or crossing the plants. In addition, the procedure could potentially be automated since no centrifugation is required.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/s001220051092</doi><tpages>3</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 1999-03, Vol.98 (3/4), p.462-464 |
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| language | eng |
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| subjects | Arabidopsis thaliana Biological and medical sciences Chromosomes Classical genetics, quantitative genetics, hybrids crossing DNA extraction Fundamental and applied biological sciences. Psychology Genetic aspects genetic markers Genetics of eukaryotes. Biological and molecular evolution Genotype identification Identification and classification inbred lines leaves microsatellite repeats Microsatellites (Genetics) pedigree Plant genetics polymerase chain reaction Pteridophyta, spermatophyta Quality control recombinant inbred lines sodium hydroxide treatment Vegetals |
| title | Fast and reliable genotype validation using microsatellite markers in Arabidopsis thaliana |
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