Fast and reliable genotype validation using microsatellite markers in Arabidopsis thaliana

In this paper we show how rogue genotypes in the parental stocks or contaminants among the crossed progeny of Arabidopsis thaliana can be readily identified and excluded from the breeding process using microsatellite markers derived from a small quantity of intact leaf tissue which has been alkali-t...

Full description

Saved in:
Bibliographic Details
Published in:Theoretical and applied genetics 1999-03, Vol.98 (3/4), p.462-464
Main Authors: Virk, P.S, Pooni, H.S, Syed, N.H, Kearsey, M.J
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Biological and medical sciences
Chromosomes
Classical genetics, quantitative genetics, hybrids
crossing
DNA
extraction
Fundamental and applied biological sciences. Psychology
Genetic aspects
genetic markers
Genetics of eukaryotes. Biological and molecular evolution
Genotype
identification
Identification and classification
inbred lines
leaves
microsatellite repeats
Microsatellites (Genetics)
pedigree
Plant genetics
polymerase chain reaction
Pteridophyta, spermatophyta
Quality control
recombinant inbred lines
sodium hydroxide treatment
Vegetals
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper we show how rogue genotypes in the parental stocks or contaminants among the crossed progeny of Arabidopsis thaliana can be readily identified and excluded from the breeding process using microsatellite markers derived from a small quantity of intact leaf tissue which has been alkali-treated. This method is fast and cost effective as it does not require DNA extraction, is highly reliable, and is less damaging to small plants where only limited quantities of plant tissue are available. Furthermore, a large number of samples can be processed in 1 day, facilitating the identification process prior to selfing or crossing the plants. In addition, the procedure could potentially be automated since no centrifugation is required.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220051092