Antimicrobial factor from Bacillus amyloliquefaciens inhibits Paenibacillus larvae, the causative agent of American foulbrood

Bacillus amyloliquefaciens LBM 5006 produces an antimicrobial factor active against Paenibacillus larvae , a major honeybee pathogen. The antagonistic effect and the mode of action of the antimicrobial factor were investigated. The antibacterial activity was produced starting at mid-logarithmic grow...

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Bibliographic Details
Published in:Archives of microbiology 2012-03, Vol.194 (3), p.177-185
Main Authors: Benitez, Lisianne Brittes, Velho, Renata Voltolini, de Souza da Motta, Amanda, Segalin, Jéferson, Brandelli, Adriano
Format: Article
Language:English
Subjects:
American foulbrood
Animals
Antibacterial activity
Antibiotics
Antifungal Agents - pharmacology
Antimicrobial activity
Antimicrobial agents
Apis mellifera
Bacillus - chemistry
Bacillus amyloliquefaciens
Bacteriology
Bees
Bees - microbiology
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Cell Biology
Cell envelopes
Cell suspensions
Ecology
Endopeptidase K
Fundamental and applied biological sciences. Psychology
Honey
Larva - growth & development
Larva - microbiology
Life Sciences
Mass Spectrometry
Mass spectroscopy
Microbial Ecology
Microbial Sensitivity Tests
Microbiology
Microscopy, Electron, Scanning
Miscellaneous
Optical density
Original Paper
Paenibacillus - drug effects
Paenibacillus - ultrastructure
Paenibacillus larvae
Pathogens
Peptides
Peptides, Cyclic - pharmacology
Scanning electron microscopy
Scientific imaging
Spores
Spores, Fungal - drug effects
stationary phase
Trypsin
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Summary:Bacillus amyloliquefaciens LBM 5006 produces an antimicrobial factor active against Paenibacillus larvae , a major honeybee pathogen. The antagonistic effect and the mode of action of the antimicrobial factor were investigated. The antibacterial activity was produced starting at mid-logarithmic growth phase, reaching its maximum during the stationary phase. Exposure of cell suspensions of P. larvae to this antimicrobial resulted in loss of cell viability and reduction in optical density associated with cell lysis. Scanning electron microscopy showed damaged cell envelope and loss of protoplasmic material. The antimicrobial factor was stable for up to 80°C, but it was sensitive to proteinase K and trypsin. Mass spectrometry analysis indicates that the antimicrobial activity is associated with iturin-like peptides. The antimicrobial factor from B. amyloliquefaciens LBM 5006 showed a bactericidal effect against P. larvae cells and spores. This is the first report on iturin activity against P. larvae . This antimicrobial presents potential for use in the control of American foulbrood disease.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-011-0743-4