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Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification
Equine sarcoids are the most common tumor of horses. Bovine papillomavirus (BPV) has been suggested as the cause of sarcoids. Studies have shown that BPV is present in swabs or biopsies from nonsarcoid-bearing equine skin. Skin biopsies from a variety of different conditions and normal skin from hor...
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| Published in: | Journal of veterinary diagnostic investigation 2012, Vol.24 (1), p.32-41 |
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| creator | Wobeser, Bruce K Hill, Janet E Jackson, Marion L Kidney, Beverly A Mayer, Monique N Townsend, Hugh G.G Allen, Andrew L |
| description | Equine sarcoids are the most common tumor of horses. Bovine papillomavirus (BPV) has been suggested as the cause of sarcoids. Studies have shown that BPV is present in swabs or biopsies from nonsarcoid-bearing equine skin. Skin biopsies from a variety of different conditions and normal skin from horses with no reported history of sarcoids were examined by polymerase chain reaction (PCR) for the presence of BPV, which was found in all different types of skin conditions as well as normal skin. Forty-one out of 86 skin biopsies from horses without sarcoids were found to contain BPV DNA. Laser microdissection, followed by DNA amplification through both PCR and isothermal loop-mediated amplification, was performed on these 41 biopsies and on 70 additional BPV-positive sarcoid biopsies to localize the virus. Location of BPV DNA was different between sarcoid and nonsarcoid groups. Nonsarcoid skin biopsies were more likely to have BPV within intact or inflamed epidermis than sarcoids (P = 0.016 and P = 0.007, respectively). Areas of inflammation within the dermis and epidermis were more likely to contain BPV than in noninflamed areas (P = 0.008 and P = 0.009, respectively). Bovine papillomavirus was also found in the epidermis of all types of sarcoids examined, more frequently in occult sarcoids than in fibroblastic and nodular types (P = 0.03 and P = 0.01, respectively). Results suggest that BPV is commonly found in normal and inflamed equine skin, and it is likely an important predisposing factor in the development of sarcoids. |
| doi_str_mv | 10.1177/1040638711425952 |
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Bovine papillomavirus (BPV) has been suggested as the cause of sarcoids. Studies have shown that BPV is present in swabs or biopsies from nonsarcoid-bearing equine skin. Skin biopsies from a variety of different conditions and normal skin from horses with no reported history of sarcoids were examined by polymerase chain reaction (PCR) for the presence of BPV, which was found in all different types of skin conditions as well as normal skin. Forty-one out of 86 skin biopsies from horses without sarcoids were found to contain BPV DNA. Laser microdissection, followed by DNA amplification through both PCR and isothermal loop-mediated amplification, was performed on these 41 biopsies and on 70 additional BPV-positive sarcoid biopsies to localize the virus. Location of BPV DNA was different between sarcoid and nonsarcoid groups. Nonsarcoid skin biopsies were more likely to have BPV within intact or inflamed epidermis than sarcoids (P = 0.016 and P = 0.007, respectively). Areas of inflammation within the dermis and epidermis were more likely to contain BPV than in noninflamed areas (P = 0.008 and P = 0.009, respectively). Bovine papillomavirus was also found in the epidermis of all types of sarcoids examined, more frequently in occult sarcoids than in fibroblastic and nodular types (P = 0.03 and P = 0.01, respectively). Results suggest that BPV is commonly found in normal and inflamed equine skin, and it is likely an important predisposing factor in the development of sarcoids.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/1040638711425952</identifier><identifier>PMID: 22362933</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Bovine papillomavirus ; Bovine papillomavirus 1 - genetics ; Dermatitis - veterinary ; Dermatitis - virology ; Dermatologic Surgical Procedures ; dermis ; DNA ; DNA, Viral - genetics ; Horse Diseases - virology ; horses ; Horses - virology ; inflammation ; Keratinocytes - virology ; Microdissection - methods ; Microdissection - veterinary ; Microscopy, Confocal - methods ; Microscopy, Confocal - veterinary ; Nucleic Acid Amplification Techniques - methods ; Nucleic Acid Amplification Techniques - veterinary ; Papillomavirus Infections - veterinary ; Papillomavirus Infections - virology ; polymerase chain reaction ; Polymerase Chain Reaction - veterinary ; sarcoid ; Sarcoidosis - veterinary ; Sarcoidosis - virology ; Skin - virology ; Skin Diseases, Viral - veterinary ; Skin Diseases, Viral - virology ; viruses</subject><ispartof>Journal of veterinary diagnostic investigation, 2012, Vol.24 (1), p.32-41</ispartof><rights>2012 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-a0047e8dca2e2abefc26ae37a265a67ab1eb5c88759a6c5471fe06decb44e64a3</citedby><cites>FETCH-LOGICAL-c434t-a0047e8dca2e2abefc26ae37a265a67ab1eb5c88759a6c5471fe06decb44e64a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,786,790,4043,27956,27957,27958</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22362933$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wobeser, Bruce K</creatorcontrib><creatorcontrib>Hill, Janet E</creatorcontrib><creatorcontrib>Jackson, Marion L</creatorcontrib><creatorcontrib>Kidney, Beverly A</creatorcontrib><creatorcontrib>Mayer, Monique N</creatorcontrib><creatorcontrib>Townsend, Hugh G.G</creatorcontrib><creatorcontrib>Allen, Andrew L</creatorcontrib><title>Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Equine sarcoids are the most common tumor of horses. Bovine papillomavirus (BPV) has been suggested as the cause of sarcoids. Studies have shown that BPV is present in swabs or biopsies from nonsarcoid-bearing equine skin. Skin biopsies from a variety of different conditions and normal skin from horses with no reported history of sarcoids were examined by polymerase chain reaction (PCR) for the presence of BPV, which was found in all different types of skin conditions as well as normal skin. Forty-one out of 86 skin biopsies from horses without sarcoids were found to contain BPV DNA. Laser microdissection, followed by DNA amplification through both PCR and isothermal loop-mediated amplification, was performed on these 41 biopsies and on 70 additional BPV-positive sarcoid biopsies to localize the virus. Location of BPV DNA was different between sarcoid and nonsarcoid groups. Nonsarcoid skin biopsies were more likely to have BPV within intact or inflamed epidermis than sarcoids (P = 0.016 and P = 0.007, respectively). Areas of inflammation within the dermis and epidermis were more likely to contain BPV than in noninflamed areas (P = 0.008 and P = 0.009, respectively). Bovine papillomavirus was also found in the epidermis of all types of sarcoids examined, more frequently in occult sarcoids than in fibroblastic and nodular types (P = 0.03 and P = 0.01, respectively). Results suggest that BPV is commonly found in normal and inflamed equine skin, and it is likely an important predisposing factor in the development of sarcoids.</description><subject>Animals</subject><subject>Bovine papillomavirus</subject><subject>Bovine papillomavirus 1 - genetics</subject><subject>Dermatitis - veterinary</subject><subject>Dermatitis - virology</subject><subject>Dermatologic Surgical Procedures</subject><subject>dermis</subject><subject>DNA</subject><subject>DNA, Viral - genetics</subject><subject>Horse Diseases - virology</subject><subject>horses</subject><subject>Horses - virology</subject><subject>inflammation</subject><subject>Keratinocytes - virology</subject><subject>Microdissection - methods</subject><subject>Microdissection - veterinary</subject><subject>Microscopy, Confocal - methods</subject><subject>Microscopy, Confocal - veterinary</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Nucleic Acid Amplification Techniques - veterinary</subject><subject>Papillomavirus Infections - veterinary</subject><subject>Papillomavirus Infections - virology</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>sarcoid</subject><subject>Sarcoidosis - veterinary</subject><subject>Sarcoidosis - virology</subject><subject>Skin - virology</subject><subject>Skin Diseases, Viral - veterinary</subject><subject>Skin Diseases, Viral - virology</subject><subject>viruses</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqFkTtz1DAUhT0MDHlATwXqqAx6yy5DQoCZHSggtedavl4UbGuja4cJP4dfirwbKCigkTQ63zl6nKJ4JvgrIZx7LbjmVlVOCC1NbeSD4ljUWpW6VvZhXme5XPWj4oTomnMjjROPiyMplZW1UsfFz030MIQfMIc4sdizN_E2TMh2sAvDEEe4DWkhFiaGN8sqECQfQ0cMpi5v9wOMI8wx3TH6likfpy6sWbSGfY2JkNhCYdqyAQgTG4NPsQtE6PdHrjHz98j6mMa95-LjGYNxN4Q--P2tnhSPehgIn97Pp8XV5dsv5-_Lzad3H87PNqXXSs8lcK4dVp0HiRJa7L20gMqBtAasg1Zga3xVOVOD9UY70SO3HfpWa7Qa1Gnx8pC7S_FmQZqbMZDHYYAJ40JNbbTVpnLy_6RUxuWBZ5IfyPxoooR9s0thhHTXCN6sFTZ_V5gtz-_Dl3bE7o_hd2cZKA8AwRab67ikKX_LvwJfHPgeYgPbFKi5-iy50Jxz53TN1S-K6rEP</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Wobeser, Bruce K</creator><creator>Hill, Janet E</creator><creator>Jackson, Marion L</creator><creator>Kidney, Beverly A</creator><creator>Mayer, Monique N</creator><creator>Townsend, Hugh G.G</creator><creator>Allen, Andrew L</creator><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>2012</creationdate><title>Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification</title><author>Wobeser, Bruce K ; Hill, Janet E ; Jackson, Marion L ; Kidney, Beverly A ; Mayer, Monique N ; Townsend, Hugh G.G ; Allen, Andrew L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-a0047e8dca2e2abefc26ae37a265a67ab1eb5c88759a6c5471fe06decb44e64a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Bovine papillomavirus</topic><topic>Bovine papillomavirus 1 - genetics</topic><topic>Dermatitis - veterinary</topic><topic>Dermatitis - virology</topic><topic>Dermatologic Surgical Procedures</topic><topic>dermis</topic><topic>DNA</topic><topic>DNA, Viral - genetics</topic><topic>Horse Diseases - virology</topic><topic>horses</topic><topic>Horses - virology</topic><topic>inflammation</topic><topic>Keratinocytes - virology</topic><topic>Microdissection - methods</topic><topic>Microdissection - veterinary</topic><topic>Microscopy, Confocal - methods</topic><topic>Microscopy, Confocal - veterinary</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Nucleic Acid Amplification Techniques - veterinary</topic><topic>Papillomavirus Infections - veterinary</topic><topic>Papillomavirus Infections - virology</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>sarcoid</topic><topic>Sarcoidosis - veterinary</topic><topic>Sarcoidosis - virology</topic><topic>Skin - virology</topic><topic>Skin Diseases, Viral - veterinary</topic><topic>Skin Diseases, Viral - virology</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wobeser, Bruce K</creatorcontrib><creatorcontrib>Hill, Janet E</creatorcontrib><creatorcontrib>Jackson, Marion L</creatorcontrib><creatorcontrib>Kidney, Beverly A</creatorcontrib><creatorcontrib>Mayer, Monique N</creatorcontrib><creatorcontrib>Townsend, Hugh G.G</creatorcontrib><creatorcontrib>Allen, Andrew L</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wobeser, Bruce K</au><au>Hill, Janet E</au><au>Jackson, Marion L</au><au>Kidney, Beverly A</au><au>Mayer, Monique N</au><au>Townsend, Hugh G.G</au><au>Allen, Andrew L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2012</date><risdate>2012</risdate><volume>24</volume><issue>1</issue><spage>32</spage><epage>41</epage><pages>32-41</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><notes>http://dx.doi.org/1177/1040638711425952</notes><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>ObjectType-Article-2</notes><notes>ObjectType-Feature-1</notes><abstract>Equine sarcoids are the most common tumor of horses. Bovine papillomavirus (BPV) has been suggested as the cause of sarcoids. Studies have shown that BPV is present in swabs or biopsies from nonsarcoid-bearing equine skin. Skin biopsies from a variety of different conditions and normal skin from horses with no reported history of sarcoids were examined by polymerase chain reaction (PCR) for the presence of BPV, which was found in all different types of skin conditions as well as normal skin. Forty-one out of 86 skin biopsies from horses without sarcoids were found to contain BPV DNA. Laser microdissection, followed by DNA amplification through both PCR and isothermal loop-mediated amplification, was performed on these 41 biopsies and on 70 additional BPV-positive sarcoid biopsies to localize the virus. Location of BPV DNA was different between sarcoid and nonsarcoid groups. Nonsarcoid skin biopsies were more likely to have BPV within intact or inflamed epidermis than sarcoids (P = 0.016 and P = 0.007, respectively). Areas of inflammation within the dermis and epidermis were more likely to contain BPV than in noninflamed areas (P = 0.008 and P = 0.009, respectively). Bovine papillomavirus was also found in the epidermis of all types of sarcoids examined, more frequently in occult sarcoids than in fibroblastic and nodular types (P = 0.03 and P = 0.01, respectively). Results suggest that BPV is commonly found in normal and inflamed equine skin, and it is likely an important predisposing factor in the development of sarcoids.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>22362933</pmid><doi>10.1177/1040638711425952</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bovine papillomavirus Bovine papillomavirus 1 - genetics Dermatitis - veterinary Dermatitis - virology Dermatologic Surgical Procedures dermis DNA DNA, Viral - genetics Horse Diseases - virology horses Horses - virology inflammation Keratinocytes - virology Microdissection - methods Microdissection - veterinary Microscopy, Confocal - methods Microscopy, Confocal - veterinary Nucleic Acid Amplification Techniques - methods Nucleic Acid Amplification Techniques - veterinary Papillomavirus Infections - veterinary Papillomavirus Infections - virology polymerase chain reaction Polymerase Chain Reaction - veterinary sarcoid Sarcoidosis - veterinary Sarcoidosis - virology Skin - virology Skin Diseases, Viral - veterinary Skin Diseases, Viral - virology viruses |
| title | Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification |
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