Influence of an m-type thioredoxin in maize on potyviral infection

Expression of many host genes can be altered during virus infection. In a previous study of sugarcane mosaic virus (SCMV) infection in maize ( Zea mays ), we observed that expression of ZmTrm2 , a gene encoding thioredoxin m , was up-regulated at about 10 days post-inoculation (dpi). In this present...

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Bibliographic Details
Published in:European journal of plant pathology 2011-10, Vol.131 (2), p.317-326
Main Authors: Shi, Yan, Qin, Yanhong, Cao, Yanyong, Sun, Hu, Zhou, Tao, Hong, Yiguo, Fan, Zaifeng
Format: Article
Language:English
Subjects:
Agriculture
Biological and medical sciences
Biomedical and Life Sciences
Cell walls
Corn
Ecology
Fundamental and applied biological sciences. Psychology
Gene expression
Life Sciences
Nicotiana tabacum
Phytopathology. Animal pests. Plant and forest protection
Plant diseases
Plant Pathology
Plant Sciences
Plant viruses and viroids
Sugarcane
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Summary:Expression of many host genes can be altered during virus infection. In a previous study of sugarcane mosaic virus (SCMV) infection in maize ( Zea mays ), we observed that expression of ZmTrm2 , a gene encoding thioredoxin m , was up-regulated at about 10 days post-inoculation (dpi). In this present study we determined that ZmTrm2 silencing in maize by virus-induced gene silencing significantly enhanced systemic SCMV infection. In contrast transient over-expression of ZmTrm2 in maize protoplasts inhibited accumulation of SCMV viral RNA. Furthermore, we found that in inoculated Nicotiana tabacum leaves transient expression of ZmTrm2 inhibited accumulation of the RNA of tobacco vein-banding mosaic virus (TVBMV), a potyvirus infecting dicotyledonous plants. Interestingly in ZmTrm2 transiently expressed N. tabacum leaves, we detected by semi-quantitative RT-PCR a reduced level of the mRNA of class I beta-1, 3-glucanase ( GluI ), a protein known to have a role in cell wall callose deposition and viral movement. Our data indicate that the maize ZmTrm2 plays an inhibitory role during infection of plants by SCMV and TVBMV.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-011-9810-6