The effect of auxin type and cytokinin concentration on callus induction and plant regeneration frequency from immature inflorescence segments of seashore paspalum (Paspalum vaginatum Swartz)

Seashore paspalum (Paspalum vaginatum Swartz) is a salt tolerant, fine textured turfgrass used on golf courses in coastal, tropical, and subtropical regions. A callus induction and plant regeneration protocol for this commercially important turfgrass species has been developed. Induction of highly r...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Plant 2008-12, Vol.44 (6), p.480-486
Main Authors: Neibaur, Isaac, Gallo, Maria, Altpeter, Fredy
Format: Article
Language:English
Subjects:
6-Dichloro-2-methoxybenzoic acid (Dicamba)
Auxin X cytokinin interaction
Auxins
Benzylaminopurine (BA)
Biomedical and Life Sciences
Callus
Cell Biology
Cytokinins
Developmental Biology
Developmental Biology/Morphogenesis
Immature inflorescence
Immatures
Inflorescences
Life Sciences
Light
Paspalum
Paspalum vaginatum
Plant Breeding/Biotechnology
Plant cells
Plant Genetics and Genomics
Plant growth
Plant Sciences
Plants
Regeneration
Seashore paspalum (Paspalum vaginatum Swartz)
Seashores
Studies
Tissue culture
Turf grasses
Turfgrasses
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Summary:Seashore paspalum (Paspalum vaginatum Swartz) is a salt tolerant, fine textured turfgrass used on golf courses in coastal, tropical, and subtropical regions. A callus induction and plant regeneration protocol for this commercially important turfgrass species has been developed. Induction of highly regenerable callus with approximately 400 shoots per cultured immature inflorescence (1 cm in length) was achieved by culturing 0.2 cm segments on media with 3 mg l⁻¹ 3,6-dichloro-2-methoxybenzoic acid (dicamba) and 0.1 or 1.0 mg l⁻¹ benzylaminopurine (BA). A multifactorial experiment demonstrated the combination of 3 mg l⁻¹ dicamba and 1.0 mg l⁻¹ BA for induction of callus resulted in 12 times higher plant regeneration frequency compared to 3 mg l⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D) alone or ten times higher plant regeneration frequency than the combination of 3 mg l⁻¹ 2,4-D and 1.0 mg l⁻¹ BA. These results are expected to support the development of a genetic transformation protocol for seashore paspalum.
ISSN:1054-5476
1475-2689
DOI:10.1007/s11627-008-9143-0