Improved Enrichment and Isolation of Polycyclic Aromatic Hydrocarbons (PAH)-Degrading Microorganisms in Soil Using Anthracene as a Model PAH

Lack of attention to soil and microbial characteristics that influence PAHs degradation has been a leading cause of failures in isolation of efficient PAH degraders and bioaugumentation processes with microbial consortia. This study compared the classic method of isolation of PAHs-degraders with a m...

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Bibliographic Details
Published in:Current microbiology 2009-06, Vol.58 (6), p.628-634
Main Authors: Jacques, Rodrigo J. S, Okeke, Benedict C, Bento, Fátima M, Peralba, Maria C. R, Camargo, Flávio A. O
Format: Article
Language:English
Subjects:
Anthracene
Anthracenes - metabolism
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Biodegradation
Biodegradation, Environmental
Biomedical and Life Sciences
Biotechnology
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Heterotrophic microorganisms
Hydrocarbons
Life Sciences
Microbiology
Microcosms
Microorganisms
Molecular Sequence Data
Polycyclic aromatic hydrocarbons
Polycyclic Aromatic Hydrocarbons - metabolism
RNA, Ribosomal, 16S - genetics
Soil
Soil Microbiology
Soil microorganisms
Soil Pollutants - metabolism
Soils
Studies
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Summary:Lack of attention to soil and microbial characteristics that influence PAHs degradation has been a leading cause of failures in isolation of efficient PAH degraders and bioaugumentation processes with microbial consortia. This study compared the classic method of isolation of PAHs-degraders with a modified method employing a pre-enrichment respirometric analysis. The modified enrichment of PAH degrading microorganisms using in vitro microcosm resulted to reduced enrichment period and more efficient PAH-degrading microbial consortia. Results indicate that natural soils with strong heterotrophic microbial activity determined through pre-enrichment analysis, are better suited for the isolation of efficient PAH degrading microorganisms with significant reduction of the enrichment period.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-009-9381-3