Ischemic preconditioning attenuates of ischemia-induced degradation of spectrin and tau: implications for ischemic tolerance

Global ischemia selectively induces CA1 neuronal death in the hippocampus. Pretreatment with non-lethal ischemia (i.e. ischemic preconditioning) prevents CA1 neuronal death induced by lethal ischemia. While ischemic tolerance is a well-known phenomenon, the underlying molecular mechanisms are not fu...

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Bibliographic Details
Published in:Neurological sciences 2011-04, Vol.32 (2), p.229-239
Main Authors: Nakajima, Takayuki, Ochi, Syoichi, Oda, Chika, Ishii, Maki, Ogawa, Kazushige
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Brain Ischemia - metabolism
Brain Ischemia - pathology
Hippocampus - metabolism
Hippocampus - pathology
Immunohistochemistry
Ischemic Preconditioning
Male
Medicine
Medicine & Public Health
Microtubule-Associated Proteins - metabolism
Neurology
Neuroradiology
Neurosciences
Neurosurgery
Original Article
Psychiatry
Rats
Rats, Sprague-Dawley
Spectrin - metabolism
tau Proteins - metabolism
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Summary:Global ischemia selectively induces CA1 neuronal death in the hippocampus. Pretreatment with non-lethal ischemia (i.e. ischemic preconditioning) prevents CA1 neuronal death induced by lethal ischemia. While ischemic tolerance is a well-known phenomenon, the underlying molecular mechanisms are not fully understood. Cytoskeletal proteins including α-spectrin, tau, and microtubule-associated protein 2 (MAP-2) are indispensable for the maintenance of neuronal homeostasis. Here, we report the effects of ischemic preconditioning on the ischemia-induced degradation of cytoskeletal proteins α-spectrin, tau, and MAP-2 in the rat CA1 region. We found that most neurons of the CA1 region had died after 5 min of ischemia. However, exposing the brain to 3 min of ischemic preconditioning 3 days earlier significantly reduced the number of neuronal death. A significant degradation of α-spectrin and tau, but not of MAP-2, was found in the CA1 region after 5 min of ischemia. Ischemic preconditioning attenuated the ischemia-induced massive degradation of α-spectrin and tau. Our results suggest that the attenuation of ischemia-induced degradation of α-spectrin and tau by ischemic preconditioning may be associated with the neuroprotective mechanism of the ischemic tolerance.
ISSN:1590-1874
1590-3478
DOI:10.1007/s10072-010-0359-5