Impact of drugs approved for treating ADHD on the cell survival and energy metabolism: an in-vitro study in human neuronal and immune cells

The oxidative and antioxidative properties of psychostimulants such as methylphenidate and amphetamine are discussed controversially. The aim of the present study was to evaluate the impact of psychostimulants and atomoxetine in different concentrations between 31.25 and 5000 ng/ml on the survival o...

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Bibliographic Details
Published in:Journal of psychopharmacology (Oxford) 2010-12, Vol.24 (12), p.1829-1833
Main Authors: Schmidt, AJ, Krieg, JC, Clement, HW, Gebhardt, S., Schulz, E., Heiser, P.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Adrenergic Uptake Inhibitors - administration & dosage
Adrenergic Uptake Inhibitors - pharmacology
Amphetamine
Amphetamine - administration & dosage
Amphetamine - pharmacology
Amphetamines
Atomoxetine Hydrochloride
ATP
Attention deficit hyperactivity disorder
Biological and medical sciences
Biotechnology
Cell Line, Tumor
Cell lines
Cell survival
Cell Survival - drug effects
Central Nervous System Stimulants - administration & dosage
Central Nervous System Stimulants - pharmacology
Dose-Response Relationship, Drug
Drug abuse
Energy metabolism
Energy Metabolism - drug effects
Humans
Immunosuppressive agents
Incubation
Medical sciences
Metabolism
Methylphenidate
Methylphenidate - administration & dosage
Methylphenidate - pharmacology
Monocytes
Monocytes - drug effects
Monocytes - metabolism
Neuroblastoma - metabolism
Neuropharmacology
Pharmacology. Drug treatments
Propylamines - administration & dosage
Propylamines - pharmacology
Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer
Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease)
Psychology. Psychoanalysis. Psychiatry
Psychopharmacology
Statistical analysis
Survival
U937 Cells
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Summary:The oxidative and antioxidative properties of psychostimulants such as methylphenidate and amphetamine are discussed controversially. The aim of the present study was to evaluate the impact of psychostimulants and atomoxetine in different concentrations between 31.25 and 5000 ng/ml on the survival of human neuronal (neuroblastoma SH-SY5Y) and immune (monocytic U-937) cells and the impact of psychostimulants and atomoxetine in different concentrations between 500 and 5000 ng/ml on energy metabolism (adenosine triphosphate [ATP] content) in SH-SY5Y cells. Statistical analysis revealed that incubation for 24 h with amphetamine led to a significantly enhanced cell survival in both cell lines after treatment with various (32.5, 125, 250 and 1250 ng/ml) concentrations. Methylphenidate and atomoxetine induced a significantly enhanced cell survival at lower concentrations in the SH-SY5Y cell line, whereas in the U-937 cell line higher concentrations increased the cell survival. Incubation with the highest concentration of methylphenidate (5000 ng/ml) caused a significant reduction of cell survival in both cell types. Measurement of ATP contents in the neuronal cell line revealed no significant effects of the investigated compounds. Our results show that the examined substances exert concentration-dependent effects on cell survival in both applied cell lines.
ISSN:0269-8811
1461-7285
DOI:10.1177/0269881109105563