Bacterial leaf blight resistance genes (Xa21, xa13 and xa5) pyramiding through molecular marker assisted selection into rice cultivars

Marker assisted selection was employed to pyramid three bacterial blight resistance genes Xa21, xa13 and xa5 into high yielding susceptible rice cultivars ADT43 and ADT47. With the assistance of PCR markers, homozygous and heterozygous genotypes were identified in F 2 generation of two crosses (ADT4...

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Bibliographic Details
Published in:Archiv für Phytopathologie und Pflanzenschutz 2010-07, Vol.43 (10), p.1032-1043
Main Authors: Bharani, M., Nagarajan, P., Rabindran, R., Saraswathi, R., Balasubramanian, P., Ramalingam, J.
Format: Article
Language:English
Subjects:
Bacteria
bacterial leaf blight
gene pyramiding
marker assisted selection
Oryza sativa
rice
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Summary:Marker assisted selection was employed to pyramid three bacterial blight resistance genes Xa21, xa13 and xa5 into high yielding susceptible rice cultivars ADT43 and ADT47. With the assistance of PCR markers, homozygous and heterozygous genotypes were identified in F 2 generation of two crosses (ADT43 × IRBB60 and ADT47 × IRBB60) and goodness of fit was tested. Eighty nine plants from F 3 generation of ADT43 × IRBB60 were also screened for resistance genes. The genotypes carrying resistance genes in different combinations were identified. The pyramided lines showed a wider spectrum and higher level of resistance against two Xoo isolates under field conditions.
ISSN:0323-5408
1477-2906
DOI:10.1080/03235400802285471