Optimization of high-efficiency transfection of adult human mesenchymal stem cells in vitro

With the advent of recent protocols to isolate multipotent human mesenchymal stem cells (MSCs), there is a need for efficient transfection methodologies for these cells. Most standard transfection methods yield poor transfection efficiencies for MSCs (

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Bibliographic Details
Published in:Molecular biotechnology 2005-05, Vol.30 (1), p.9-20
Main Authors: Haleem-Smith, Hana, Derfoul, Assia, Okafor, Chukwuka, Tuli, Richard, Olsen, Douglas, Hall, David J, Tuan, Rocky S
Format: Article
Language:English
Subjects:
Adult
Bone marrow
Bone Marrow Cells - cytology
Bone Marrow Cells - physiology
Cartilage Oligomeric Matrix Protein
Cell Differentiation - genetics
Cell Separation - methods
Cells, Cultured
Collagen Type II - biosynthesis
Collagen Type II - genetics
Cytomegalovirus
Efficiency
Electroporation - methods
Extracellular Matrix Proteins - genetics
Flow Cytometry - methods
Gene Expression
Glycoproteins - genetics
Humans
Matrilin Proteins
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Multipotent Stem Cells - cytology
Multipotent Stem Cells - physiology
Optimization
Plasmids - genetics
Promoter Regions, Genetic - genetics
Proteins
Stem cells
Studies
Transfection - methods
Citations: Items that cite this one
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Description
Summary:With the advent of recent protocols to isolate multipotent human mesenchymal stem cells (MSCs), there is a need for efficient transfection methodologies for these cells. Most standard transfection methods yield poor transfection efficiencies for MSCs (
ISSN:1073-6085
1559-0305
1073-6085
DOI:10.1385/mb:30:1:009