Principles and practices of laser scanning confocal microscopy

The laser scanning confocal microscope (LSCM) is an essential tool for many biomedical imaging applications at the level of the light microscope. The basic principles of confocal microscopy and the evolution of the LSCM into today's sophisticated instruments are outlined. The major imaging mode...

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Bibliographic Details
Published in:Molecular biotechnology 2000-10, Vol.16 (2), p.127-149
Main Author: PADDOCK, Stephen W
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Butterflies
Cell Nucleus - ultrastructure
Drosophila - embryology
Fluorescent Antibody Technique
Fluorescent Dyes - pharmacology
Fundamental and applied biological sciences. Psychology
Image Processing, Computer-Assisted
Lasers
Microscopy
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Molecular biophysics
Principles
Software
Spectroscopy : techniques and spectras
Studies
Time Factors
Wings, Animal - ultrastructure
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Description
Summary:The laser scanning confocal microscope (LSCM) is an essential tool for many biomedical imaging applications at the level of the light microscope. The basic principles of confocal microscopy and the evolution of the LSCM into today's sophisticated instruments are outlined. The major imaging modes of the LSCM are introduced including single optical sections, multiple wavelength images, three-dimensional reconstructions, and living cell and tissue sequences. Practical aspects of specimen preparation, image collection, and image presentation are included along with a primer on troubleshooting the LSCM for the novice.
ISSN:1073-6085
1559-0305
1073-6085
DOI:10.1385/mb:16:2:127