Microwave-assisted enzymatic synthesis of beef tallow biodiesel

Optimal conditions for the microwave-assisted enzymatic synthesis of biodiesel have been developed by a full 22 factorial design leading to a set of seven runs with different combinations of molar ratio and temperature. The main goal was to reduce the reaction time preliminarily established by a pro...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2012-04, Vol.39 (4), p.529-536
Main Authors: Da Rós, Patrícia C. M, de Castro, Heizir F, Carvalho, Ana K. F, Soares, Cleide M. F, de Moraes, Flavio F, Zanin, Gisella M
Format: Article
Language:English
Subjects:
Animals
Biocatalysis
Biochemistry
Bioconversions. Hemisynthesis
biodiesel
Biodiesel fuels
Biofuel production
Biofuels
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Burkholderia cepacia
Burkholderia cepacia - enzymology
Catalysis
Cattle
Energy
Enzymes
Enzymes, Immobilized
esters
Ethanol
Fats - metabolism
Fatty acids
Fundamental and applied biological sciences. Psychology
Genetic Engineering
heat treatment
Industrial applications and implications. Economical aspects
Industrial Microbiology
Inorganic Chemistry
Life Sciences
Lipase - chemistry
Lipase - metabolism
Margarine
Methods. Procedures. Technologies
Microbiology
Microwaves
Molecular weight
Oils & fats
Polyethylene glycol
Polyvinyl alcohol
Raw materials
Studies
substrate specificity
tallow
Temperature
triacylglycerol lipase
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Summary:Optimal conditions for the microwave-assisted enzymatic synthesis of biodiesel have been developed by a full 22 factorial design leading to a set of seven runs with different combinations of molar ratio and temperature. The main goal was to reduce the reaction time preliminarily established by a process of conventional heating. Reactions yielding biodiesel, in which beef tallow and ethanol used as raw materials were catalyzed by lipase from Burkholderia cepacia immobilized on silica-PVA and microwave irradiations within the range of 8–15 W were performed to reach the reaction temperature. Under optimized conditions (1:6 molar ratio of beef tallow to ethanol molar ratio at 50°C) almost total conversion of the fatty acid presented in the original beef tallow was converted into ethyl esters in a reaction that required 8 h, i.e., a productivity of about 92 mg ethyl esters g−1 h−1. This represents an increase of sixfold for the process carried out under conventional heating. In general, the process promises low energy demand and higher biodiesel productivity. The microwave assistance speeds up the enzyme catalyzed reactions, decreases the destructive effects on the enzyme of the operational conditions such as, higher temperature, stability, and specificity to its substrate, and allows the entire reaction medium to be heated uniformly.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-011-1059-8