Iron, Copper, and Zinc Transport: Inhibition of Divalent Metal Transporter 1 (DMT1) and Human Copper Transporter 1 (hCTR1) by shRNA

Iron (Fe), copper (Cu), and zinc (Zn) fulfill various essential biological functions and are vital for all living organisms. They play important roles in oxygen transport, cell growth and differentiation, neurotransmitter synthesis, myelination, and synaptic transmission. Because of their role in ma...

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Bibliographic Details
Published in:Biological trace element research 2012-05, Vol.146 (2), p.281-286
Main Authors: Espinoza, Alejandra, Le Blanc, Solange, Olivares, Manuel, Pizarro, Fernando, Ruz, Manuel, Arredondo, Miguel
Format: Article
Language:English
Subjects:
active transport
Biochemistry
Biological Transport - genetics
Biological Transport - physiology
Biomedical and Life Sciences
Biotechnology
Blotting, Western
Caco-2 Cells
Cation Transport Proteins - genetics
Cation Transport Proteins - metabolism
Cation Transport Proteins - physiology
cell growth
Copper
Copper - metabolism
Copper - pharmacokinetics
Dietary minerals
Endosomal Sorting Complexes Required for Transport
food fortification
Functional foods & nutraceuticals
human cell lines
Humans
Iron
Iron - metabolism
Iron - pharmacokinetics
Life Sciences
myelination
Nutrition
Oncology
oxygen
plasmids
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleic acid
RNA
RNA Interference
RNA, Small Interfering - genetics
synaptic transmission
Transfection
Zinc
Zinc - metabolism
Zinc - pharmacokinetics
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Summary:Iron (Fe), copper (Cu), and zinc (Zn) fulfill various essential biological functions and are vital for all living organisms. They play important roles in oxygen transport, cell growth and differentiation, neurotransmitter synthesis, myelination, and synaptic transmission. Because of their role in many critical functions, they are commonly used in food fortification and supplementation strategies globally. To determine the involvement of divalent metal transporter 1 (DMT1) and human copper transporter 1 (hCTR1) on Fe, Cu, and Zn uptake, Caco-2 cells were transfected with four different shRNA plasmids to selectively inhibit DMT1 or hCTR1 transporter expression. Fe and Cu uptake and total Zn content measurements were performed in shRNA-DMT1 and shRNA-hCTR1 cells. Both shRNA-DMT1 and shRNA-hCTR1 cells had lower apical Fe uptake (a decrease of 51% and 41%, respectively), Cu uptake (a decrease of 25.8% and 38.5%, respectively), and Zn content (a decrease of 23.1% and 22.7%, respectively) compared to control cells. These results confirm that DMT1 is involved in active transport of Fe, Cu, and Zn although Zn showed a different relative capacity. These results also show that hCTR1 is able to transport Fe and Zn.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-011-9243-2