DNA extraction from dried Schistosoma haematobium eggs isolated on nylon filters

Genetic studies on Schistosoma haematobium are often carried out on DNA extracted from miracidia, cercariae or adult worms. This paper presents a method for extracting DNA from S. haematobium eggs collected from urine samples and stored on nylon filters at room temperature. DNA was extracted from dr...

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Bibliographic Details
Published in:	Transactions of the Royal Society of Tropical Medicine and Hygiene 2012-04, Vol.106 (4), p.270-272
Main Authors:	Reinstrup, L., Jørgensen, A., Vennervald, B.J., Kristensen, T.K.
Format:	Article
Language:	eng
Subjects:	Animals Biological and medical sciences Child DNA extraction DNA, Helminth - isolation & purification Egg Female Filtration General aspects Genetic variation Humans Male Medical sciences Mozambique - epidemiology Nylon filters Parasite Egg Count Polymerase Chain Reaction - instrumentation Schistosoma haematobium Schistosoma haematobium - genetics Schistosoma haematobium - isolation & purification Schistosomiasis haematobia - epidemiology Schistosomiasis haematobia - urine Schools Sensitivity and Specificity Species Specificity Students - statistics & numerical data Urine filtration
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Summary:	Genetic studies on Schistosoma haematobium are often carried out on DNA extracted from miracidia, cercariae or adult worms. This paper presents a method for extracting DNA from S. haematobium eggs collected from urine samples and stored on nylon filters at room temperature. DNA was extracted from dried S. haematobium eggs using the DNeasy Blood & Tissue Kit (QIAGEN Sample & Assay Technologies, Copenhagen, Denmark). Selected genes were amplified using PCR to verify that DNA extraction had been successful. DNA was extracted from 45 samples and 31 had a positive PCR reaction for either or both of the two selected genes.
ISSN:	0035-9203 1878-3503