Origin and immunophenotype of aberrant IEL in RCDII patients

► Aberrant intra-epithelial lymphocytes (IELs) are the hallmark of refractory coeliac disease type II RCDII. ► Our data indicates that aberrant IELs in duodenal biopsies of RCDII patients originate from deranged developing precursor T-lymphocytes, while the various populations display heterogeneous...

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Bibliographic Details
Published in:Molecular immunology 2012-04, Vol.50 (4), p.262-270
Main Authors: Tack, Greetje J., van Wanrooij, Roy L.J., Langerak, Anton W., Tjon, Jennifer M.L., von Blomberg, B. Mary E., Heideman, Danielle A.M., van Bergen, Jeroen, Koning, Frits, Bouma, Gerd, Mulder, Chris J.J., Schreurs, Marco W.J.
Format: Article
Language:English
Subjects:
Aberrant lymphocytes
Aged
Biopsy
Blotting, Southern
Celiac disease
Celiac Disease - immunology
Celiac Disease - pathology
Cell Separation
Cytotoxicity
Differentiation
Enteropathy associated T-cell lymphoma
Female
Flow Cytometry
Gene Rearrangement, T-Lymphocyte - genetics
Gene Rearrangement, T-Lymphocyte - immunology
granzyme B
Humans
Immunophenotyping
Intestinal Mucosa - immunology
Intestinal Mucosa - pathology
Lymphocytes - immunology
Lymphocytes - pathology
Lymphocytes T
Male
Maturity
Middle Aged
Origin
Proliferating cell nuclear antigen
Receptors, Antigen, T-Cell - genetics
Receptors, Antigen, T-Cell - immunology
Refractory coeliac disease
Surface Plasmon Resonance
T-cell lymphoma
T-cell receptor
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Summary:► Aberrant intra-epithelial lymphocytes (IELs) are the hallmark of refractory coeliac disease type II RCDII. ► Our data indicates that aberrant IELs in duodenal biopsies of RCDII patients originate from deranged developing precursor T-lymphocytes, while the various populations display heterogeneous TCR gene rearrangements, but all contain high levels of granzyme B. ► Only patients harboring the most mature aberrant IEL population developed an EATL. ► TCR-beta gene rearrangement analysis in addition to phenotypical identification of aberrant IELs could be useful to identify at risk aberrant IEL population. Aberrant intra-epithelial lymphocytes (IELs) are the hallmark of refractory coeliac disease type II RCDII and considered a premalignant cell population from which aggressive enteropathy-associated T cell lymphoma (EATL) can evolve. The aim of this study was to gain further insight in the origin and characteristics of aberrant IELs by analysing T-cell receptor (TCR) rearrangements, and by immunophenotypic analysis of aberrant IELs. Duodenal biopsies from 18 RCDII patients and three RCDII cell lines were analysed for the presence of TCR delta, gamma, and beta rearrangements. In addition, IELs isolated from biopsies derived from RCDII patients were phenotypically analysed. Aberrant IELs showed an upregulated expression of granzyme B and decreased expression of PCNA. TCR rearrangements in the aberrant IEL population in biopsies of RCDII patients were heterogenic, which is most likely due to a variation in maturity. Similarly, RCDII cell lines displayed a heterogenic TCR rearrangement pattern. Aberrant IELs originate from deranged immature T lymphocytes and display clear differentiation to a cytotoxic phenotype. Aberrant IELs displayed different stages of maturity between RCDII patients, of which only the patients harbouring the most mature aberrant IEL population developed an EATL.
ISSN:0161-5890
1872-9142
DOI:10.1016/j.molimm.2012.01.014