12-hydroxy-eicosatetraenoic acid (12-HETE): a biomarker of Churg-Strauss syndrome

Summary Background Churg‐Strauss syndrome (CSS) shares similarities with asthma and hypereosinophilic syndrome (HES). Eicosanoids – important inflammatory and signaling molecules – are present in exhaled breath condensate (EBC) and broncho‐alveolar lavage fluid (BALF). Objectives To assess eicosanoi...

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Bibliographic Details
Published in:Clinical and experimental allergy 2012-04, Vol.42 (4), p.513-522
Main Authors: Szczeklik, W., Sanak, M., Mastalerz, L., Sokołowska, B. M., Gielicz, A., Soja, J., Kumik, J., Musiał, J., Szczeklik, A.
Format: Article
Language:English
Subjects:
12-HETE
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - analysis
Adult
Asthma
Biological and medical sciences
biomarkers
Biomarkers - analysis
Breath Tests
Bronchoalveolar Lavage Fluid - chemistry
Chromatography
Chromatography, High Pressure Liquid
Churg-Strauss syndrome
Churg-Strauss Syndrome - diagnosis
Churg-Strauss Syndrome - metabolism
Condensates
eicosanoids
Exhalation
exhaled breath condensate
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
High-performance liquid chromatography
Humans
Inflammation
Leukocytes (eosinophilic)
Male
Mass spectroscopy
Medical sciences
Metabolites
Middle Aged
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Tandem Mass Spectrometry
vasculitis
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Summary:Summary Background Churg‐Strauss syndrome (CSS) shares similarities with asthma and hypereosinophilic syndrome (HES). Eicosanoids – important inflammatory and signaling molecules – are present in exhaled breath condensate (EBC) and broncho‐alveolar lavage fluid (BALF). Objectives To assess eicosanoid profile both in EBC and BALF of CSS subjects searching for a pattern characteristic of this syndrome. Methods EBCs from 23 CSS patients, 30 asthmatics, 12 HES patients and 54 healthy controls (HC) were assessed quantitatively for 19 eicosanoids by a high‐performance liquid chromatography ‐ tandem mass spectrometry (HPLC‐MS/MS). In addition, in 21 of 23 CSS subjects and in nine asthmatics, eicosanoids were determined in BALF. Results EBC from CSS patients showed markedly elevated levels of 12‐HETE as compared with other studied groups. BALF was characterized by a significant elevation of 12‐HETE and its metabolite 12‐tetranor HETE in CSS as compared with asthma. Clinical activity of CSS correlated with 12‐HETE and its metabolites levels in BALF, but not in EBC. Conclusion and Clinical Relevance CSS is clearly distinguished from bronchial asthma, and HES by a marked increase in 12‐HETE concentration in both EBC and BALF. This points to a possible new pathogenic mechanism in CSS and may help in future in establishing the diagnosis of CSS.
ISSN:0954-7894
1365-2222
DOI:10.1111/j.1365-2222.2011.03943.x